| A controlled study of colonic immune activity and beta7+ blood T lymphocytes in patients with irritable bowel syndrome. | |
| | |
MedLine Citation:
|
PMID: 16234043 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
BACKGROUND & AIMS: The mechanisms behind irritable bowel syndrome (IBS) are incompletely understood. Recently several studies have suggested a low-grade colonic inflammation as initiator of the gut dysfunctions recorded in this patient group. The aim of this study was to characterize the phenotype and homing properties of colonic and peripheral blood lymphocytes in patients with IBS. METHODS: Patients with IBS (n=33), defined by the Rome II criteria, were compared with UC patients (n=23) and control subjects (n=15) without gastrointestinal symptoms. Colonic and peripheral blood lymphocytes were analyzed by flow cytometry. Secretion of IFN-gamma from intestinal biopsies was determined by enzyme-linked immunosorbent assay, and immunohistochemical staining of colonic biopsies was performed. RESULTS: IBS patients displayed an increased frequency of peripheral blood CD4+ and CD8+ T cells expressing the gut homing integrin beta7. Accordingly, IBS and UC patients had an augmented frequency of lamina propria CD8+ T cells in the ascending colon as compared with control subjects. The frequency of intestinal T cells expressing integrin beta7+ was unaltered in IBS and UC patients, although the expression of mucosal addressin cell adhesion molecule-1+ endothelium, the ligand for integrin beta7, was increased in the ascending colon of IBS and UC patients as compared with control subjects. CONCLUSIONS: Patients with IBS exhibit an enhanced immune activity in the gut and an increased frequency of integrin beta7+ T lymphocytes in the peripheral blood. Our data further support the hypothesis of IBS being at least partially an inflammatory disorder. |
| | |
Authors:
|
Lena Ohman; Stefan Isaksson; Anna Lundgren; Magnus Simrén; Henrik Sjövall |
Related Documents
:
|
1173693 - Proximal small bowel transit pattern in patients with malabsorption induced by bovine m... 3538693 - Zuelzer-wilson's syndrome and absence of the enteric nervous system. two rare forms of ... 22852003 - Systemic inflammation in peripheral arterial disease with or without coexistent chronic... |
Publication Detail:
|
Type: Clinical Trial; Comparative Study; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: Clinical gastroenterology and hepatology : the official clinical practice journal of the American Gastroenterological Association Volume: 3 ISSN: 1542-3565 ISO Abbreviation: Clin. Gastroenterol. Hepatol. Publication Date: 2005 Oct |
Date Detail:
|
Created Date: 2005-10-19 Completed Date: 2005-11-29 Revised Date: 2008-11-21 |
Medline Journal Info:
|
Nlm Unique ID: 101160775 Medline TA: Clin Gastroenterol Hepatol Country: United States |
Other Details:
|
Languages: eng Pagination: 980-6 Citation Subset: IM |
Affiliation:
|
Department of Internal Medicine, The Sahlgrenska Academy at Göteborg University, Göteborg, Sweden. lena.ohman@microbio.gu.se |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Adult CD8-Positive T-Lymphocytes / immunology Colitis, Ulcerative / immunology Enzyme-Linked Immunosorbent Assay Female Flow Cytometry Humans Immunohistochemistry Integrin beta Chains / analysis* Interferon-gamma / analysis Intestinal Mucosa / immunology Irritable Bowel Syndrome / immunology* Male T-Lymphocytes / immunology* |
| Chemical | |
Reg. No./Substance:
|
0/Integrin beta Chains; 0/integrin beta7; 82115-62-6/Interferon-gamma |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Endoscopic ultrasound-guided trucut biopsy of the cyst wall for diagnosing cystic pancreatic tumors.
Next Document: Effect of nitazoxanide in persistent diarrhea and enteritis associated with Blastocystis hominis.