Document Detail

A controlled randomized trial evaluating the effect of lowered incubator oxygen tension on live births in a predominantly blastocyst transfer program.
MedLine Citation:
PMID:  18927130     Owner:  NLM     Status:  MEDLINE    
BACKGROUND: The potentially damaging effect of free O(2) radicals to cultured embryos may be reduced by adding scavengers to the culture media or by reducing the incubator O(2) levels. However, lowering the O(2) in the culture environment can be expensive, troublesome and may not be justifiable. The objective of this study was to evaluate the effect of lowered incubator O(2) tension on live birth rates in a predominately Day 5 embryo transfer program. METHODS: Two hundred and thirty first-cycle women undergoing routine IVF or ICSI with ejaculated sperm were randomized in a prospective clinical trial and stratified for patient age and physician. Embryos of patients were randomly assigned for culture in either a 21% O(2) (atmospheric) or 5% O(2) (reduced) environment. Clinical endpoints monitored were rates of implantation, clinical pregnancy, live birth and blastocyst cryopreservation. RESULTS: Embryos cultured in a 5% O(2) environment consistently resulted in higher rates of live birth implantation (106/247, 42.9% versus 82/267, 30.7%; difference of 12.2% with 95% confidence interval (CI) of 3.9-20.3, P = 0.005) and live births (66/115, 57.4% versus 49/115, 42.6%; difference of 14.8% with 95% CI of 1.9-27.0, P = 0.043) when compared with rates among women whose embryos were cultured in an atmospheric O(2) environment. CONCLUSIONS: The overall increase in live births demonstrated by this study indicates that the effort and expense to culture embryos in a low-O(2) environment is justified. The study was registered at NCT00708487.
Marius Meintjes; Samuel J Chantilis; James D Douglas; Alfred J Rodriguez; Ali R Guerami; David M Bookout; Brian D Barnett; James D Madden
Publication Detail:
Type:  Journal Article; Randomized Controlled Trial     Date:  2008-10-16
Journal Detail:
Title:  Human reproduction (Oxford, England)     Volume:  24     ISSN:  1460-2350     ISO Abbreviation:  Hum. Reprod.     Publication Date:  2009 Feb 
Date Detail:
Created Date:  2009-01-16     Completed Date:  2009-04-15     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8701199     Medline TA:  Hum Reprod     Country:  England    
Other Details:
Languages:  eng     Pagination:  300-7     Citation Subset:  IM    
Frisco Institute for Reproductive Medicine, Frisco, TX 75034, USA.
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MeSH Terms
Blastocyst / drug effects*
Embryo Culture Techniques / methods*
Embryo Transfer*
Fertilization in Vitro
Live Birth*
Oxygen / administration & dosage,  chemistry,  pharmacology*
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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