Document Detail

The control of lipid metabolism by mRNA splicing in Drosophila.
MedLine Citation:
PMID:  24333419     Owner:  NLM     Status:  Publisher    
The storage of lipids is an evolutionarily conserved process that is important for the survival of organisms during shifts in nutrient availability. Triglycerides are stored in lipid droplets, but the mechanisms of how lipids are stored in these structures are poorly understood. Previous in vitro RNAi screens have implicated several components of the spliceosome in controlling lipid droplet formation and storage, but the in vivo relevance of these phenotypes is unclear. In this study, we identify specific members of the splicing machinery that are necessary for normal triglyceride storage in the Drosophila fat body. Decreasing the expression of the splicing factors U1-70K, U2AF38, U2AF50 in the fat body resulted in decreased triglyceride levels. Interestingly, while decreasing the SR protein 9G8 in the larval fat body yielded a similar triglyceride phenotype, its knockdown in the adult fat body resulted in a substantial increase in lipid stores. This increase in fat storage is due in part to altered splicing of the gene for the β-oxidation enzyme CPT1, producing an isoform with less enzymatic activity. Together, these data indicate a role for mRNA splicing in regulating lipid storage in Drosophila and provide a link between the regulation of gene expression and lipid homeostasis.
Robert M Gingras; Michelle E Warren; Alexis A Nagengast; Justin R Diangelo
Related Documents :
12271199 - Solving the problem of weight control for tube-fed patients.
23402719 - Chronic intake of a cafeteria diet and subsequent abstinence. sex-specific effects on g...
19497659 - Ansbbr applied to the treatment of wastewater from a personal care industry: effect of ...
21300159 - Influence of diet enriched with green tea on innate humoral and cellular immune respons...
24639419 - Regular intake of high-oleic peanuts improves fat oxidation and body composition in ove...
25080539 - Postnatal prebiotic fiber intake in offspring exposed to gestational protein restrictio...
3842859 - The effects of different levels of dietary lead on zinc metabolism in dairy calves.
7412559 - Site differences in fat cells of new zealand obese mice--a transplantation study.
11377129 - Vitamin and mineral supplement use by healthy teenagers in korea: motivating factors an...
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2013-12-11
Journal Detail:
Title:  Biochemical and biophysical research communications     Volume:  -     ISSN:  1090-2104     ISO Abbreviation:  Biochem. Biophys. Res. Commun.     Publication Date:  2013 Dec 
Date Detail:
Created Date:  2013-12-17     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0372516     Medline TA:  Biochem Biophys Res Commun     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2013. Published by Elsevier Inc.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Imaging Axl expression in pancreatic and prostate cancer xenografts.
Next Document:  8-Oxo-2'-deoxyguanosine ameliorates features of metabolic syndrome in obese mice.