Document Detail


The contribution of sampling uncertainty to total measurement uncertainty in the enumeration of microorganisms in foods.
MedLine Citation:
PMID:  22365349     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
Random samples of each of several food products were obtained from defined lots during processing or from retail outlets. The foods included raw milk (sampled on farm and from a bulk-milk tanker), sprouted seeds, raw minced meat, frozen de-shelled raw prawns, neck-flaps from raw chicken carcasses and ready-to-eat sandwiches. Duplicate sub-samples, generally of 100 g, were examined for aerobic colony counts; some were examined also for counts of presumptive Enterobacteriaceae and campylobacters. After log(10)-transformation, all sets of colony count data were evaluated for conformity with the normal distribution (ND) and analysed by standard ANOVA and a robust ANOVA to determine the relative contributions of the variance between and within samples to the overall variance. Sampling variance accounted for >50% of the reproducibility variance for the majority of foods examined; in many cases it exceeded 85%. We also used an iterative procedure of re-sampling without replacement to determine the effects of sample size (i.e. the number of samples) on the precision of the estimate of variance for one of the larger data sets. The variance of the repeatability and reproducibility variances depended on the number of replicate samples tested (n) in a manner that was characteristic of the underlying distribution. The results are discussed in relation to the use of measurement uncertainty in assessing compliance of results with microbiological criteria for foods.
Authors:
Basil Jarvis; Alan J Hedges; Janet E L Corry
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Publication Detail:
Type:  Journal Article     Date:  2012-01-14
Journal Detail:
Title:  Food microbiology     Volume:  30     ISSN:  1095-9998     ISO Abbreviation:  Food Microbiol.     Publication Date:  2012 Jun 
Date Detail:
Created Date:  2012-02-27     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8601127     Medline TA:  Food Microbiol     Country:  England    
Other Details:
Languages:  eng     Pagination:  362-71     Citation Subset:  IM    
Copyright Information:
Copyright © 2012 Elsevier Ltd. All rights reserved.
Affiliation:
Ross Biosciences Ltd, Upton Bishop, Ross-on-Wye, HR9 7UR, UK.
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