Document Detail


The contribution of neutrophil-derived myeloperoxidase in the early phase of fulminant acute respiratory distress syndrome induced by influenza virus infection.
MedLine Citation:
PMID:  22211924     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Because the pathogenesis of acute respiratory distress syndrome (ARDS) induced by influenza virus infection remains unknown, we can only improve on existing therapeutic interventions. To approach the subject, we investigated immunological etiology focused on cytokines and an acute lung damage factor in influenza-induced ARDS by using a PR-8 (A/H1N1)-infected mouse model. The infected mouse showed fulminant severe pneumonia with leukocyte infiltration, claudin alteration on broncheolar epithelial tight junctions, and formation of hyaline membranes. In addition to IFN-α, plenty of keratinocyte-derived chemokine (KC), macrophage inflammatory protein 2 (MIP-2), regulated on activation, normal T cell expressed and secreted (RANTES), and monocyte chemotactic protein 1 (MCP-1) were significantly released into bronchoalveolar lavage fluid (BALF) of the model. We focused on neutrophil myeloperoxidase (MPO) as a potent tissue damage factor and examined its contribution in influenza pneumonia by using mice genetically lacking in MPO. The absence of MPO reduced inflammatory damage with suppression of leakage of total BALF proteins associated with alteration of claudins in the lung. MPO(-/-) mice also suppressed viral load in the lung. This study suggests that MPO-mediated OCl(-) generation affects claudin molecules and leads to protein leakage and viral spread as a damage factor oin influenza-induced ARDS.
Authors:
Ryuichi Sugamata; Hideki Dobashi; Tomokazu Nagao; Ki-Ichi Yamamoto; Noriko Nakajima; Yuko Sato; Yasuaki Aratani; Masamichi Oshima; Tetsutaro Sata; Kazuo Kobayashi; Shoji Kawachi; Toshinori Nakayama; Kazuo Suzuki
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2011-12-30
Journal Detail:
Title:  Microbiology and immunology     Volume:  -     ISSN:  1348-0421     ISO Abbreviation:  -     Publication Date:  2011 Dec 
Date Detail:
Created Date:  2012-1-3     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7703966     Medline TA:  Microbiol Immunol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
© 2011 The Societies and Blackwell Publishing Asia Pty Ltd.
Affiliation:
Inflammation Program, Department of Immunology, Graduate School of Medicine, Chiba University, Inohana 1-8-1, Chuo-ku, Chiba-city, Chiba 260-8670 Japan Departments of Immunology, National Institute of Infectious Diseases, Toyama 1-23-1, Shinjuku-ku, Tokyo 162-8640, Japan Departments of Pathology, National Institute of Infectious Diseases, Toyama 1-23-1, Shinjuku-ku, Tokyo 162-8640, Japan Graduate School of Nanobioscience, Yokohama City University, Seto 22-2, Kanazawa-ku, Yokohama-city, Kanagawa 236-0027, Japan Division of Anesthesia, Surgical Operation Department, National Center for Global Health and Medicine, Toyama 1-21-1, Shinjuku-ku, Tokyo 162-8655, Japan. Department of Immunology, Graduate School of Medicine, Chiba University, Inohana 1-8-1, Chuo-ku, Chiba-city, Chiba 260-8670 Japan.
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