| The contribution of endothelial cells to hyperacute rejection in xenogeneic perfused working hearts. | |
| | |
MedLine Citation:
|
PMID: 8310518 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
The mechanisms leading to the hyperacute rejection of a vascularized xenograft are still incompletely understood. The first stage of the rejection process is when blood of the recipient comes into contact with the endothelium of the xenograft. A working heart model was used to examine endothelium-related processes and their impact on organ function. Pig hearts were perfused with porcine (autologous) or human (xenogeneic) blood. Cardiac function was evaluated by calculating the stroke work index, arteriovenous oxygen, coronary flow, and resistance. PgF1a as a marker of endothelial activation, its antagonist TXB2, and myoglobin reflecting myocardial damage were measured in the hemoperfusate. H&E and PAS staining and immunohistological demonstration of factor VIII-related antigen was performed. Xenogeneic perfused porcine hearts showed significantly less stroke work, a higher arteriovenous oxygen difference, and an increased coronary resistance. Factor VIII-related antigen could not be demonstrated immunohistologically on the endothelium after xenogeneic perfusion. PgF1a levels were significantly higher in the xenogeneic hemoperfusate, indicating endothelial cell activation. The concentration of myoglobin in the hemoperfusate remained within normal values and was similar during autologous and xenogeneic perfusion. Therefore endothelium-related processes are likely to affect the coronary circulation--thus being one mechanism leading to diminished cardiac performance during hyperacute rejection. |
| | |
Authors:
|
M M Suckfüll; O Pieske; M Müdsam; R Babic; C Hammer |
Related Documents
:
|
9160868 - The role of na+-h+ exchange occurring during hypoxia in the genesis of reoxygenation-in... 7271668 - Hemodynamic changes during whole body surface cooling and lower body negative pressure. 20154598 - Effects of cerebrovascular pressure reactivity-guided optimization of cerebral perfusio... 2306468 - Loss of cardiac contractility and severe morphologic changes by acutely lowering the ph... 9160868 - The role of na+-h+ exchange occurring during hypoxia in the genesis of reoxygenation-in... 12015748 - Reduction of hypoxia-inducible heme oxygenase-1 in the myocardium after left ventricula... |
Publication Detail:
|
Type: Journal Article |
Journal Detail:
|
Title: Transplantation Volume: 57 ISSN: 0041-1337 ISO Abbreviation: Transplantation Publication Date: 1994 Jan |
Date Detail:
|
Created Date: 1994-03-16 Completed Date: 1994-03-16 Revised Date: 2004-11-17 |
Medline Journal Info:
|
Nlm Unique ID: 0132144 Medline TA: Transplantation Country: UNITED STATES |
Other Details:
|
Languages: eng Pagination: 262-7 Citation Subset: IM |
Affiliation:
|
Institute for Surgical Research, Ludwigs-Maximilians University, Munich, Germany. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Acute Disease Animals Blood / immunology Endothelium, Vascular / physiopathology* Factor VIII / metabolism Female Graft Rejection / physiopathology* Heart Function Tests Heart Transplantation / pathology, physiology* Humans Male Myoglobin / blood Perfusion Prostaglandins F / blood Swine Thromboxane B2 / blood Transplantation, Heterologous / immunology* |
| Chemical | |
Reg. No./Substance:
|
0/Myoglobin; 0/Prostaglandins F; 54397-85-2/Thromboxane B2; 745-62-0/prostaglandin F1; 9001-27-8/Factor VIII |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Induction of specific unresponsiveness to cardiac allografts by short-term administration of anti-T ...
Next Document: Evidence that the antibiotic ciprofloxacin counteracts cyclosporine-dependent suppression of cytokin...