Document Detail

The contribution of endothelial cells to hyperacute rejection in xenogeneic perfused working hearts.
MedLine Citation:
PMID:  8310518     Owner:  NLM     Status:  MEDLINE    
The mechanisms leading to the hyperacute rejection of a vascularized xenograft are still incompletely understood. The first stage of the rejection process is when blood of the recipient comes into contact with the endothelium of the xenograft. A working heart model was used to examine endothelium-related processes and their impact on organ function. Pig hearts were perfused with porcine (autologous) or human (xenogeneic) blood. Cardiac function was evaluated by calculating the stroke work index, arteriovenous oxygen, coronary flow, and resistance. PgF1a as a marker of endothelial activation, its antagonist TXB2, and myoglobin reflecting myocardial damage were measured in the hemoperfusate. H&E and PAS staining and immunohistological demonstration of factor VIII-related antigen was performed. Xenogeneic perfused porcine hearts showed significantly less stroke work, a higher arteriovenous oxygen difference, and an increased coronary resistance. Factor VIII-related antigen could not be demonstrated immunohistologically on the endothelium after xenogeneic perfusion. PgF1a levels were significantly higher in the xenogeneic hemoperfusate, indicating endothelial cell activation. The concentration of myoglobin in the hemoperfusate remained within normal values and was similar during autologous and xenogeneic perfusion. Therefore endothelium-related processes are likely to affect the coronary circulation--thus being one mechanism leading to diminished cardiac performance during hyperacute rejection.
M M Suckfüll; O Pieske; M Müdsam; R Babic; C Hammer
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Transplantation     Volume:  57     ISSN:  0041-1337     ISO Abbreviation:  Transplantation     Publication Date:  1994 Jan 
Date Detail:
Created Date:  1994-03-16     Completed Date:  1994-03-16     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  0132144     Medline TA:  Transplantation     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  262-7     Citation Subset:  IM    
Institute for Surgical Research, Ludwigs-Maximilians University, Munich, Germany.
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MeSH Terms
Acute Disease
Blood / immunology
Endothelium, Vascular / physiopathology*
Factor VIII / metabolism
Graft Rejection / physiopathology*
Heart Function Tests
Heart Transplantation / pathology,  physiology*
Myoglobin / blood
Prostaglandins F / blood
Thromboxane B2 / blood
Transplantation, Heterologous / immunology*
Reg. No./Substance:
0/Myoglobin; 0/Prostaglandins F; 54397-85-2/Thromboxane B2; 745-62-0/prostaglandin F1; 9001-27-8/Factor VIII

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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