| The contribution of the capillary endothelium to blood clearance and tissue deposition of anionic quantum dots in vivo. | |
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MedLine Citation:
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PMID: 20619783 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The increasing interest in biomedical applications of semiconductor quantum dots (QDs) is closely linked to the use of surface modifications to target specific sites of the body. The immense surface area of vascular endothelium is a possible interaction platform with systemically administered QDs. Therefore, the aim of this study was to investigate the microvascular distribution of neutral, cationic, and anionic QDs in vivo. QDs with carboxyl-, amine- and polyethylene glycol surface coatings were injected into the blood circulation of mice. In vivo microscopy of the cremaster muscle, two-photon microscopy of skeletal and heart muscle, as well as quantitative fluorescence measurements of blood, excreta, and tissue samples were performed. Transmission electron microscopy was used to detect QDs at the cellular level. The in vitro association of QDs with cultured endothelial cells was investigated by flow cytometry and confocal microscopy. Anionic QDs exhibited a very low residence time in the blood stream, preferably accumulated in organs with a prominent mononuclear phagocytic component, but were also found in other tissues with low phagocytic properties where they were predominantly associated with capillary endothelium. This deposition behavior was identified as a new, phagocyte-independent principle contributing to the rapid clearance of anionic QDs from the circulation. |
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Authors:
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Marc Praetner; Markus Rehberg; Peter Bihari; Max Lerchenberger; Bernd Uhl; Martin Holzer; Martin E Eichhorn; Robert Fürst; Tamara Perisic; Christoph A Reichel; Ulrich Welsch; Fritz Krombach |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-06-17 |
Journal Detail:
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Title: Biomaterials Volume: 31 ISSN: 1878-5905 ISO Abbreviation: Biomaterials Publication Date: 2010 Sep |
Date Detail:
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Created Date: 2010-07-12 Completed Date: 2010-10-20 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8100316 Medline TA: Biomaterials Country: England |
Other Details:
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Languages: eng Pagination: 6692-700 Citation Subset: IM |
Copyright Information:
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Copyright 2010 Elsevier Ltd. All rights reserved. |
Affiliation:
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Walter Brendel Centre of Experimental Medicine, Ludwig-Maximilians-Universität München, Munich 81377, Germany. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Anions Endothelial Cells / cytology, metabolism, ultrastructure Endothelium, Vascular / metabolism* Hemodynamics Injections, Intra-Arterial Kinetics Male Mice Mice, Inbred C57BL Microscopy Microvessels / cytology, metabolism Muscle, Skeletal / blood supply, cytology, metabolism Myocardium / cytology, metabolism, ultrastructure Photons Quantum Dots* Tissue Distribution |
| Chemical | |
Reg. No./Substance:
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0/Anions |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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