Document Detail


The contribution of DNA repair and antioxidants in determining cell type-specific resistance to oxidative stress.
MedLine Citation:
PMID:  17050169     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The aims of this study were; (i) to elucidate the mechanisms involved in determining cell type-specific responses to oxidative stress and (ii) to test the hypothesis that cell types which are subjected to high oxidative burdens in vivo, have greater oxidative stress resistance. Cultures of the retinal pigment epithelium (RPE), corneal fibroblasts, alveolar type II epithelium and skin epidermal cells were studied. Cellular sensitivity to H2O2 was determined by the MTT assay. Cellular antioxidant status (CuZnSOD, MnSOD, GPX, CAT) was analyzed with enzymatic assays and the susceptibility and repair capacities of nuclear and mitochondrial genomes were assessed by QPCR. Cell type-specific responses to H2O2 were observed. The RPE had the greatest resistance to oxidative stress (P>0.05; compared to all other cell types) followed by the corneal fibroblasts (P < 0.05; compared to skin and lung cells). The oxidative tolerance of the RPE coincided with greater CuZnSOD, GPX and CAT enzymatic activity (P < 0.05; compared to other cells). The RPE and corneal fibroblasts both had up-regulated nDNA repair post-treatment (P < 0.05; compared to all other cells). In summary, variations in the synergistic interplay between enzymatic antioxidants and nDNA repair have important roles in influencing cell type-specific vulnerability to oxidative stress. Furthermore, cells located in highly oxidizing microenvironments appear to have more efficient oxidative defence and repair mechanisms.
Authors:
Stuart G Jarrett; Julie Albon; Mike Boulton
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Free radical research     Volume:  40     ISSN:  1071-5762     ISO Abbreviation:  Free Radic. Res.     Publication Date:  2006 Nov 
Date Detail:
Created Date:  2006-10-19     Completed Date:  2007-02-07     Revised Date:  2007-08-13    
Medline Journal Info:
Nlm Unique ID:  9423872     Medline TA:  Free Radic Res     Country:  England    
Other Details:
Languages:  eng     Pagination:  1155-65     Citation Subset:  IM    
Affiliation:
Cell and Molecular Biology Unit, School of Optometry and Vision Sciences, Cardiff University, Cardiff, UK.
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MeSH Terms
Descriptor/Qualifier:
Antioxidants / chemistry*,  metabolism
Catalase / metabolism
Cell Line
Cell Survival
DNA Damage
DNA Repair*
DNA, Mitochondrial / metabolism
Humans
Hydrogen Peroxide / pharmacology
Mitosis
Oxidative Stress*
Oxygen / metabolism
Superoxide Dismutase / metabolism
Tetrazolium Salts / pharmacology
Thiazoles / pharmacology
Time Factors
Grant Support
ID/Acronym/Agency:
//Wellcome Trust
Chemical
Reg. No./Substance:
0/Antioxidants; 0/DNA, Mitochondrial; 0/Tetrazolium Salts; 0/Thiazoles; 298-93-1/thiazolyl blue; 7722-84-1/Hydrogen Peroxide; 7782-44-7/Oxygen; EC 1.11.1.6/Catalase; EC 1.15.1.1/Superoxide Dismutase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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