Document Detail

Scar/WAVE3 contributes to motility and plasticity of lamellipodial dynamics but not invasion in three dimensions.
MedLine Citation:
PMID:  22909346     Owner:  NLM     Status:  MEDLINE    
The Scar (suppressor of cAMP receptor)/WAVE [WASP (Wiskott-Aldrich syndrome protein) verprolin homologous] complex plays a major role in the motility of cells by activating the Arp2/3 complex, which initiates actin branching and drives protrusions. Mammals have three Scar/WAVE isoforms, which show some tissue-specific expression, but their functions have not been differentiated. In the present study we show that depletion of Scar/WAVE3 in the mammalian breast cancer cells MDA-MB-231 results in larger and less dynamic lamellipodia. Scar/WAVE3-depleted cells move more slowly but more persistently on a two-dimensional matrix and they typically only show one lamellipod. However, Scar/WAVE3 appears to have no role in driving invasiveness in a three-dimensional Matrigel™ invasion assay or a three-dimensional collagen invasion assay, suggesting that lamellipodial persistence as seen in two-dimensions is not crucial in three-dimensional environments.
Heather J Spence; Paul Timpson; Hao Ran Tang; Robert H Insall; Laura M Machesky
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Publication Detail:
Type:  In Vitro; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Biochemical journal     Volume:  448     ISSN:  1470-8728     ISO Abbreviation:  Biochem. J.     Publication Date:  2012 Nov 
Date Detail:
Created Date:  2012-10-18     Completed Date:  2013-01-14     Revised Date:  2014-10-07    
Medline Journal Info:
Nlm Unique ID:  2984726R     Medline TA:  Biochem J     Country:  England    
Other Details:
Languages:  eng     Pagination:  35-42     Citation Subset:  IM    
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MeSH Terms
Adenocarcinoma / pathology
Breast Neoplasms / pathology
Cell Line, Tumor / cytology,  ultrastructure
Cell Movement
Cell Shape
Clone Cells / cytology
Drug Combinations
Neoplasm Invasiveness
Neoplasm Proteins / antagonists & inhibitors,  genetics,  physiology*
Pseudopodia / chemistry,  physiology*,  ultrastructure
RNA Interference
RNA, Small Interfering / pharmacology
Time-Lapse Imaging
Wiskott-Aldrich Syndrome Protein Family / antagonists & inhibitors,  genetics,  physiology*
Grant Support
15673//Cancer Research UK; A10298//Cancer Research UK; //Cancer Research UK
Reg. No./Substance:
0/Drug Combinations; 0/Gels; 0/Laminin; 0/Neoplasm Proteins; 0/Proteoglycans; 0/RNA, Small Interfering; 0/WASF3 protein, human; 0/Wiskott-Aldrich Syndrome Protein Family; 119978-18-6/matrigel; 9007-34-5/Collagen

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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