Document Detail

A continuous lineage of rat adenohypophysis stromal cells: characterisation and effects on GH(3)B(6) prolactin-secreting cell behaviour.
MedLine Citation:
PMID:  12566225     Owner:  NLM     Status:  MEDLINE    
Although some studies have shown a possible modulation of the stroma on the hormonal secretion, it is not clear as to what are the requirements for these cellular interactions. In the present work, a homogeneous and continuous lineage of rat adenohypophysis stromal cells (APS9 cells) obtained from rat adenohypophysis primary culture was established. Using immunocytochemical methods and electron microscopy, we have characterised APS9 cells as elongated fibroblastoid-like cells with intercellular contacts, expressing alpha-smooth muscle actin, type IV collagen and laminin. By biochemical procedures, higher amounts of chondroitin sulphate and heparan sulphate were found in the pericellular and extracellular compartments of APS9 cell culture. In order to evaluate the possible effects of APS9 cell on GH(3)B(6) prolactin-secreting cell survival and/or proliferation, we established co-culture and proliferation assays. When GH(3)B(6) cells were cultivated on APS9 cell substrate, they displayed an organisation of many cellular cords strongly attached and covering all the stromal cell area, establishing punctual interactions or extensive surface associations between adjacent cells. Prolactin immunoreactivity appeared to be more scattered throughout the cytoplasm and accumulated in its periphery. When plated on glass coverslips, on newborn rat skin fibroblasts, on murine haematopoietic bone marrow stroma cell line or on murine foetal liver stroma cell line, GH(3)B(6) cells changed their organisation and presented a decrease in cell number and adherence to the substrate. Our results showed that the APS9 cell/GH(3)B(6) cell interactions favour cell growth and probably PRL secretion, and raises questions about the specificity of different organs and/or animal species stromas on the hormone secretion.
Leandro M Alves; Cláudio C Werneck; Rita de Cássia L Martins Rd; Luiz Cláudio F Silva; Marília Taffarel; Radovan Borojevic; Luiz E Nasciutti
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biology of the cell / under the auspices of the European Cell Biology Organization     Volume:  94     ISSN:  0248-4900     ISO Abbreviation:  Biol. Cell     Publication Date:  2002 Nov 
Date Detail:
Created Date:  2003-02-04     Completed Date:  2003-11-06     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8108529     Medline TA:  Biol Cell     Country:  France    
Other Details:
Languages:  eng     Pagination:  519-33     Citation Subset:  IM    
Departamento de Histologia e Embriologia, Instituto de Ciências Biomédicas, Universidade Federal do Rio de Janeiro, Cidade Universitária - Ilha do Fundão 21941-590, Rio de Janeiro, Brazil.
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MeSH Terms
Cell Communication / physiology*
Cell Division
Cell Line
Cell Lineage
Cell Size
Coculture Techniques
Microscopy, Fluorescence
Pituitary Gland, Anterior / cytology*,  physiology
Prolactin / secretion*
Rats, Wistar
Stromal Cells / cytology*,  physiology
Reg. No./Substance:

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