Document Detail


A conformation-specific anti-peptide antibody to the beta-type platelet-derived growth factor receptor also recognizes the activated epidermal growth factor receptor.
MedLine Citation:
PMID:  7713896     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Earlier we reported the generation of a conformation-specific antibody (Ab P2) to the beta-type platelet-derived growth factor receptor (Bishayee, S., Majumdar, S., Scher, C. D., and Khan, S. (1988) Mol. Cell. Biol. 8, 3696-3702). Ab P2 is directed to a 16-amino acid peptide (Glu-Gly-Tyr-Lys-Lys-Lys-Tyr-Gln-Gln-Val-Asp-Glu-Glu-Phe-Leu-Arg) of the cytoplasmic domain of the receptor, and it recognizes the phosphorylated platelet-derived growth factor receptor but not the unphosphorylated receptor. We now report that Ab P2 also interacts with the epidermal growth factor receptor and that the recognition is specific for a conformation induced by phosphorylation of the receptor; however, Ab P2 is not directed to phosphotyrosine. Studies conducted with P2-derived peptides suggest that the conformation-specific antibody is directed to an acidic tripeptide, Asp-Glu-Glu, and this sequence is also present in the cytoplasmic domain of the epidermal growth factor receptor. With respect to the C terminus amino acid or ATP-binding site, Asp-Glu-Glu is located in different regions in these receptors; nevertheless, this tripeptide along with the surrounding amino acids is cryptic in the unphosphorylated receptor, and tyrosine phosphorylation uncovers this site. This suggests that the Asp-Glu-Glu sequence may be important in receptor functions.
Authors:
K Panneerselvam; H Reitz; S A Khan; S Bishayee
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Journal of biological chemistry     Volume:  270     ISSN:  0021-9258     ISO Abbreviation:  J. Biol. Chem.     Publication Date:  1995 Apr 
Date Detail:
Created Date:  1995-05-15     Completed Date:  1995-05-15     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  2985121R     Medline TA:  J Biol Chem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  7975-9     Citation Subset:  IM    
Affiliation:
Coriell Institute for Medical Research, Camden, New Jersey 08103, USA.
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Sequence
Animals
Antibodies / immunology*
Binding Sites, Antibody
Cell Line
Cross Reactions
Humans
Mice
Molecular Sequence Data
Peptides / immunology*
Phosphorylation
Protein Conformation
Receptor, Epidermal Growth Factor / immunology*,  metabolism
Receptors, Platelet-Derived Growth Factor / immunology*,  metabolism
Tumor Cells, Cultured
Chemical
Reg. No./Substance:
0/Antibodies; 0/Binding Sites, Antibody; 0/Peptides; EC 2.7.10.1/Receptor, Epidermal Growth Factor; EC 2.7.10.1/Receptors, Platelet-Derived Growth Factor

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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