Document Detail


Confocal laser scanning microscopy and ultrastructural study of VGLUT2 thalamic input to striatal projection neurons in rats.
MedLine Citation:
PMID:  23047588     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We examined thalamic input to striatum in rats using immunolabeling for the vesicular glutamate transporter (VGLUT2). Double immunofluorescence viewed with confocal laser scanning microscopy (CLSM) revealed that VGLUT2+ terminals are distinct from VGLUT1+ terminals. CLSM of Phaseolus vulgaris-leucoagglutinin (PHAL)-labeled cortical or thalamic terminals revealed that VGLUT2 is rare in corticostriatal terminals but nearly always present in thalamostriatal terminals. Electron microscopy revealed that VGLUT2+ terminals made up 39.4% of excitatory terminals in striatum (with VGLUT1+ corticostriatal terminals constituting the rest), and 66.8% of VGLUT2+ terminals synapsed on spines and the remainder on dendrites. VGLUT2+ axospinous terminals had a mean diameter of 0.624 μm, while VGLUT2+ axodendritic terminals a mean diameter of 0.698 μm. In tissue in which we simultaneously immunolabeled thalamostriatal terminals for VGLUT2 and striatal neurons for D1 (with about half of spines immunolabeled for D1), 54.6% of VGLUT2+ terminals targeted D1+ spines (i.e., direct pathway striatal neurons), and 37.3% of D1+ spines received VGLUT2+ synaptic contacts. By contrast, 45.4% of VGLUT2+ terminals targeted D1-negative spines (i.e., indirect pathway striatal neurons), and only 25.8% of D1-negative spines received VGLUT2+ synaptic contacts. Similarly, among VGLUT2+ axodendritic synaptic terminals, 59.1% contacted D1+ dendrites, and 40.9% contacted D1-negative dendrites. VGLUT2+ terminals on D1+ spines and dendrites tended to be slightly smaller than those on D1-negative spines and dendrites. Thus, thalamostriatal terminals contact both direct and indirect pathway striatal neurons, with a slight preference for direct. These results are consistent with physiological studies indicating slightly different effects of thalamic input on the two types of striatal projection neurons.
Authors:
Wanlong Lei; Yunping Deng; Bingbing Liu; Shuhua Mu; Natalie M Guley; Ting Wong; Anton Reiner
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Journal of comparative neurology     Volume:  521     ISSN:  1096-9861     ISO Abbreviation:  J. Comp. Neurol.     Publication Date:  2013 Apr 
Date Detail:
Created Date:  2013-02-21     Completed Date:  2013-10-29     Revised Date:  2014-08-27    
Medline Journal Info:
Nlm Unique ID:  0406041     Medline TA:  J Comp Neurol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1354-77     Citation Subset:  IM    
Copyright Information:
Copyright © 2012 Wiley Periodicals, Inc.
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MeSH Terms
Descriptor/Qualifier:
Animals
Corpus Striatum / chemistry,  cytology,  ultrastructure*
Excitatory Postsynaptic Potentials / physiology
Male
Microscopy, Confocal / methods
Neural Pathways / chemistry,  cytology,  ultrastructure
Neurons / chemistry,  ultrastructure*
Rats
Rats, Sprague-Dawley
Thalamus / chemistry,  cytology,  ultrastructure*
Vesicular Glutamate Transport Protein 2 / physiology,  ultrastructure*
Grant Support
ID/Acronym/Agency:
NS-19620/NS/NINDS NIH HHS; NS-28721/NS/NINDS NIH HHS; NS-57722/NS/NINDS NIH HHS; R01 NS057722/NS/NINDS NIH HHS
Chemical
Reg. No./Substance:
0/Slc17a6 protein, rat; 0/Vesicular Glutamate Transport Protein 2
Comments/Corrections

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