Document Detail


ON cone bipolar cell axonal synapses in the OFF inner plexiform layer of the rabbit retina.
MedLine Citation:
PMID:  23042441     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Analysis of the rabbit retinal connectome RC1 reveals that the division between the ON and the OFF inner plexiform layer (IPL) is not structurally absolute. ON cone bipolar cells make noncanonical axonal synapses onto specific targets and receive amacrine cell synapses in the nominal OFF layer, creating novel motifs, including inhibitory crossover networks. Automated transmission electron microscopic imaging, molecular tagging, tracing, and rendering of ~400 bipolar cells reveals axonal ribbons in 36% of ON cone bipolar cells, throughout the OFF IPL. The targets include γ-aminobutyrate (GABA)-positive amacrine cells (γACs), glycine-positive amacrine cells (GACs), and ganglion cells. Most ON cone bipolar cell axonal contacts target GACs driven by OFF cone bipolar cells, forming new architectures for generating ON-OFF amacrine cells. Many of these ON-OFF GACs target ON cone bipolar cell axons, ON γACs, and/or ON-OFF ganglion cells, representing widespread mechanisms for OFF to ON crossover inhibition. Other targets include OFF γACs presynaptic to OFF bipolar cells, forming γAC-mediated crossover motifs. ON cone bipolar cell axonal ribbons drive bistratified ON-OFF ganglion cells in the OFF layer and provide ON drive to polarity-appropriate targets such as bistratified diving ganglion cells (bsdGCs). The targeting precision of ON cone bipolar cell axonal synapses shows that this drive incidence is necessarily a joint distribution of cone bipolar cell axonal frequency and target cell trajectories through a given volume of the OFF layer. Such joint distribution sampling is likely common when targets are sparser than sources and when sources are coupled, as are ON cone bipolar cells.
Authors:
J Scott Lauritzen; James R Anderson; Bryan W Jones; Carl B Watt; Shoeb Mohammed; John V Hoang; Robert E Marc
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  The Journal of comparative neurology     Volume:  521     ISSN:  1096-9861     ISO Abbreviation:  J. Comp. Neurol.     Publication Date:  2013 Apr 
Date Detail:
Created Date:  2013-01-30     Completed Date:  2013-08-21     Revised Date:  2014-04-02    
Medline Journal Info:
Nlm Unique ID:  0406041     Medline TA:  J Comp Neurol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  977-1000     Citation Subset:  IM    
Copyright Information:
Copyright © 2012 Wiley Periodicals, Inc.
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MeSH Terms
Descriptor/Qualifier:
Amacrine Cells / metabolism,  ultrastructure
Animals
Animals, Newborn
Axons / metabolism,  ultrastructure
Dendrites / metabolism,  ultrastructure
Electron Microscope Tomography
Eye Proteins / metabolism
Female
Glutamic Acid / metabolism
Glycine / metabolism
Image Processing, Computer-Assisted
Models, Neurological
Rabbits
Retina / anatomy & histology*
Retinal Bipolar Cells / classification,  metabolism,  physiology*
Retinal Ganglion Cells / physiology,  ultrastructure
Synapses / metabolism,  physiology*,  ultrastructure
Taurine / metabolism
Visual Pathways / physiology*,  ultrastructure
gamma-Aminobutyric Acid / metabolism
Grant Support
ID/Acronym/Agency:
EY014800/EY/NEI NIH HHS; EY015128/EY/NEI NIH HHS; EY02576/EY/NEI NIH HHS; P30 EY014800/EY/NEI NIH HHS; R01 EY002576/EY/NEI NIH HHS; R01 EY015128/EY/NEI NIH HHS
Chemical
Reg. No./Substance:
0/Eye Proteins; 1EQV5MLY3D/Taurine; 3KX376GY7L/Glutamic Acid; 56-12-2/gamma-Aminobutyric Acid; TE7660XO1C/Glycine
Comments/Corrections

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