Document Detail


A conducting salt-based amperometric biosensor for measurement of extracellular lactate accumulation in ischemic myocardium.
MedLine Citation:
PMID:  9230678     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In this paper, fabrication, characterization, and physiological application of a miniaturized amperometric lactate biosensor are described. The sensor is based on cross-linked lactate oxidase and tetrathiafulvalene-tetracyano-quinodimethane (TTF-TCNQ) charge transfer complex. The sensor was developed for continuous quantitative measurement of the lactate accumulation in ischemic myocardium under severe depletion of oxygen. The sensor was evaluated in vitro at an applied potential of 0.15 V vs Ag/AgCl; it proved to combine all the performance characteristics desired for the present application, such as proper response in absence of oxygen, good operational stability, good accuracy and precision (103.5 +/- 1.2%), adequate response time (t95% = 80 s), and wide linear dynamic range up to 27 mM (r = 0.9998) in N2-saturated solutions and at 37 degrees C. The prepared sensors (n = 12) showed sensitivity of 380 +/- 90 nA/mM, and a background current of 240 +/- 50 nA. The lower limit of detection is 0.4 +/- 0.15 mM with a S/N ratio equal to 3. Results obtained for direct lactate monitoring in ischemic rabbit papillary muscle under no-flow conditions and PO2 < 6 mm Hg are presented.
Authors:
S A Marzouk; V V Cosofret; R P Buck; H Yang; W E Cascio; S S Hassen
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Analytical chemistry     Volume:  69     ISSN:  0003-2700     ISO Abbreviation:  Anal. Chem.     Publication Date:  1997 Jul 
Date Detail:
Created Date:  1997-10-20     Completed Date:  1997-10-20     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0370536     Medline TA:  Anal Chem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  2646-52     Citation Subset:  IM    
Affiliation:
Department of Chemistry, University of North Carolina, Chapel Hill 27599-3290, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Biosensing Techniques*
Heart Ventricles / chemistry
Humans
Hydrogen-Ion Concentration
Lactic Acid / analysis*,  blood
Myocardial Ischemia / metabolism*
Myocardium / metabolism*
Oxygen
Rabbits
Salts
Temperature
Time Factors
Grant Support
ID/Acronym/Agency:
P01 HL27430/HL/NHLBI NIH HHS; R01 HL48769/HL/NHLBI NIH HHS
Chemical
Reg. No./Substance:
0/Salts; 50-21-5/Lactic Acid; 7782-44-7/Oxygen

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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