| A computational model for nitric oxide, nitrite and nitrate biotransport in the microcirculation: effect of reduced nitric oxide consumption by red blood cells and blood velocity. | |
| | |
MedLine Citation:
|
PMID: 20888842 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Bioavailability of vasoactive endothelium-derived nitric oxide (NO) in vasculature is a critical factor in regulation of many physiological processes. Consumption of NO by RBC plays a crucial role in maintaining NO bioavailability. Recently, Deonikar and Kavdia (2009b) reported an effective NO-RBC reaction rate constant of 0.2×10(5)M(-1)s(-1) that is ~7 times lower than the commonly used NO-RBC reaction rate constant of 1.4×10(5)M(-1)s(-1). To study the effect of lower NO-RBC reaction rate constant and nitrite and nitrate formation (products of NO metabolism in blood), we developed a 2D mathematical model of NO biotransport in 50 and 200μm ID arterioles to calculate NO concentration in radial and axial directions in the vascular lumen and vascular wall of the arterioles. We also simulated the effect of blood velocity on NO distribution in the arterioles to determine whether NO can be transported to downstream locations in the arteriolar lumen. The results indicate that lowering the NO-RBC reaction rate constant increased the NO concentration in the vascular lumen as well as the vascular wall. Increasing the velocity also led to increase in NO concentration. We predict increased NO concentration gradient along the axial direction with an increase in the velocity. The predicted NO concentration was 281-1163nM in the smooth muscle cell layer for 50μm arteriole over the blood velocity range of 0.5-4cms(-1) for k(NO-RBC) of 0.2×10(5)M(-1)s(-1), which is much higher than the reported values from earlier mathematical modeling studies. The NO concentrations are similar to the experimentally measured vascular wall NO concentration range of 300-1000nM in several different vascular beds. The results are significant from the perspective that the downstream transport of NO is possible under the right circumstances. |
| | |
Authors:
|
Prabhakar Deonikar; Mahendra Kavdia |
Publication Detail:
|
Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't Date: 2010-10-01 |
Journal Detail:
|
Title: Microvascular research Volume: 80 ISSN: 1095-9319 ISO Abbreviation: Microvasc. Res. Publication Date: 2010 Dec |
Date Detail:
|
Created Date: 2010-11-15 Completed Date: 2011-02-25 Revised Date: 2011-12-21 |
Medline Journal Info:
|
Nlm Unique ID: 0165035 Medline TA: Microvasc Res Country: United States |
Other Details:
|
Languages: eng Pagination: 464-76 Citation Subset: IM |
Copyright Information:
|
Copyright © 2010 Elsevier Inc. All rights reserved. |
Affiliation:
|
Department of Biomedical Engineering, Wayne State University, Detroit, MI 48202, USA. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Arterioles / anatomy & histology, metabolism* Biological Transport Blood Flow Velocity Computer Simulation* Erythrocytes / metabolism* Humans Kinetics Microcirculation* Models, Cardiovascular* Muscle, Smooth, Vascular / metabolism Nitrates / blood* Nitric Oxide / blood* Nitrites / blood* Numerical Analysis, Computer-Assisted |
| Grant Support | |
ID/Acronym/Agency:
|
R01 HL084337/HL/NHLBI NIH HHS; R01 HL084337-01A2/HL/NHLBI NIH HHS; R01 HL084337-05/HL/NHLBI NIH HHS; R15 HL087287/HL/NHLBI NIH HHS; R15 HL087287-01A2/HL/NHLBI NIH HHS |
| Chemical | |
Reg. No./Substance:
|
0/Nitrates; 0/Nitrites; 10102-43-9/Nitric Oxide |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Molecular mechanisms of HIV-1 mother-to-child transmission and infection in neonatal target cells.
Next Document: Transplantation of endothelial progenitor cells overexpressing endothelial nitric oxide synthase enh...