Document Detail

A comprehensive survey of the laminins and collagens type IV expressed in mouse Leydig cells and their regulation by LH/hCG.
MedLine Citation:
PMID:  18367508     Owner:  NLM     Status:  MEDLINE    
Extracellular matrix (ECM) proteins have been shown to alter Leydig cell steroidogenesis in vitro, substantiating the hypothesis that Leydig cell steroidogenic activity and matrix environment are interdependent events. However, the nature of the ECM components synthesized by Leydig cells and their regulation by LH/human chorionic gonadotropin (hCG) remain unknown. Here, we examine the occurrence of the 11 laminin subunits and the 6 alpha chains of collagen IV (COL4A1-6) by RT-PCR in Leydig cells cultured with or without LH/hCG. Leydig cells were a tumor Leydig cell line (mLTC-1) or 8-week-old mice Leydig cells. Based on PCR data, it is suggested that normal Leydig cells may synthesize a maximum of 11 laminin heterotrimers and the 6 alpha chains of collagen IV. They also may synthesize various proteases and inhibitors of the metzincin family. The mLTC-1 cells have a limited repertoire as compared with normal Leydig cells. Interestingly, none of the ten proteases and inhibitors monitored is under LH-hCG regulation whereas every protease and inhibitor of the serine protease family yet identified in Leydig cells is under gonadotropin regulation. In addition, a few laminin and collagen subunit genes are regulated by LH/hCG. These are laminins alpha3 and gamma3 (Lama3 and Lamc3), Col4a3, and Col4a6, which are negatively regulated by LH/hCG in both Leydig cell types, and Col4a4, which was downregulated in primary cultures but not in mLTC-1 cells. Collectively, the present study suggests that Leydig cells modulate in a selective fashion their matrix environment in response to their trophic hormone. This may alter the steroidogenic outcome of Leydig cells.
Séverine Mazaud Guittot; Adélie Vérot; Fanny Odet; Marie-Agnès Chauvin; Brigitte le Magueresse-Battistoni
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Reproduction (Cambridge, England)     Volume:  135     ISSN:  1741-7899     ISO Abbreviation:  Reproduction     Publication Date:  2008 Apr 
Date Detail:
Created Date:  2008-03-27     Completed Date:  2008-08-19     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  100966036     Medline TA:  Reproduction     Country:  England    
Other Details:
Languages:  eng     Pagination:  479-88     Citation Subset:  IM    
Inserm U418, UCBL1, UMR INRA 1245, Hopital Debrousse, 29 rue soeur Bouvier, 69322 Lyon Cedex 05, France.
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MeSH Terms
Cell Line
Cells, Cultured
Chorionic Gonadotropin / pharmacology
Collagen Type IV / genetics*
DNA Primers / genetics
Extracellular Matrix / drug effects,  metabolism*
Gene Expression
Laminin / genetics*
Leydig Cells / drug effects,  metabolism*
Luteinizing Hormone / pharmacology
Mice, Inbred Strains
Protein Isoforms / genetics*
Reverse Transcriptase Polymerase Chain Reaction / methods
Reg. No./Substance:
0/Chorionic Gonadotropin; 0/Collagen Type IV; 0/DNA Primers; 0/Laminin; 0/Protein Isoforms; 9002-67-9/Luteinizing Hormone

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