Document Detail


A comprehensive method for determination of fatty acids in the initial oral biofilm (pellicle).
MedLine Citation:
PMID:  22829652     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The acquired pellicle is a tenacious organic layer covering the surface of teeth, protecting the underlying dental hard tissues. Lipids account for about one quarter of the pellicle's dry weight and are assumed to be of considerable importance for their protective properties. Nevertheless, only preliminary information is available about the nature of lipids in the pellicle. Gas chromatography coupled with electron impact ionization mass spectrometry was used to establish a convenient analytical protocol in order to obtain a qualitative and quantitative characterization of a wide range of FAs (C(12)-C(22)). In situ biofilm formation was performed on bovine enamel slabs mounted on individual splints carried by 10 subjects. A modified Folch extraction procedure was adopted to extract the lipids from the detached pellicle, followed by transesterification to fatty acid methyl esters using methanol and concentrated hydrochloric acid. Tridecanoic and nonadecanoic acid were used as internal standards suitable and reliable for robust, precise and accurate measurements. The present study demonstrates, for the first time, a procedure based on a combination of innovative specimen generation and convenient sample preparation with sensitive GC-MS analysis for the determination of the fatty acid profile of the initial oral biofilm.
Authors:
Marco Reich; Christian Hannig; Ali Al-Ahmad; Richard Bolek; Klaus Kümmerer
Publication Detail:
Type:  Evaluation Studies; Journal Article; Research Support, Non-U.S. Gov't; Validation Studies     Date:  2012-07-24
Journal Detail:
Title:  Journal of lipid research     Volume:  53     ISSN:  0022-2275     ISO Abbreviation:  J. Lipid Res.     Publication Date:  2012 Oct 
Date Detail:
Created Date:  2012-09-07     Completed Date:  2013-01-29     Revised Date:  2013-10-10    
Medline Journal Info:
Nlm Unique ID:  0376606     Medline TA:  J Lipid Res     Country:  United States    
Other Details:
Languages:  eng     Pagination:  2226-30     Citation Subset:  IM    
Affiliation:
Institute of Sustainable and Environmental Chemistry, Faculty of Sustainability, Leuphana University Lüneburg, Lüneburg, Germany. marco.reich@leuphana.de
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MeSH Terms
Descriptor/Qualifier:
Animals
Biofilms*
Cattle
Dental Pellicle / chemistry*
Esterification
Fatty Acids / analysis*
Gas Chromatography-Mass Spectrometry
Chemical
Reg. No./Substance:
0/Fatty Acids
Comments/Corrections

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