Document Detail


The complement C1qA enhances retinoic acid-inducible gene-I-mediated immune signalling.
MedLine Citation:
PMID:  22260551     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The cellular innate immune response is essential for recognizing and defending against viral infection. Retinoic acid-inducible gene-I (RIG-I) and virus-induced signaling adaptor (VISA) mediated immune signalling is critically involved in RNA-virus-induced innate immune responses. Here we demonstrate that the complement C1qA interacts with different RIG-I pathway components and enhances RIG-I-VISA-mediated signalling pathway as well as TBK1-mediated activation of interferon-β (IFN-β) promoter. Our data show that over-expression of C1qA up-regulates RIG-I-mediated activation of IFN-stimulated responsive element (ISRE) and nuclear factor-κB reporters and IFN-β transcription, but not IFN regulatory factor-3-mediated and inhibitor of κB kinase-mediated activation of ISRE and nuclear factor-κB promoter. In addition, C1qA can counteract the function of the C1q receptor gC1qR in RIG-I-mediated signalling. Our results reveal the important role of complement C1qA in the innate immune response.
Authors:
Yetao Wang; Xiaomei Tong; Junjie Zhang; Xin Ye
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Immunology     Volume:  136     ISSN:  1365-2567     ISO Abbreviation:  Immunology     Publication Date:  2012 May 
Date Detail:
Created Date:  2012-04-04     Completed Date:  2012-05-16     Revised Date:  2013-05-02    
Medline Journal Info:
Nlm Unique ID:  0374672     Medline TA:  Immunology     Country:  England    
Other Details:
Languages:  eng     Pagination:  78-85     Citation Subset:  IM    
Copyright Information:
© 2012 The Authors. Immunology © 2012 Blackwell Publishing Ltd.
Affiliation:
Centre for Molecular Immunology, CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, China.
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Line
Complement C1q / immunology*
DEAD-box RNA Helicases / genetics,  immunology*,  metabolism
Dogs
Humans
Immunity, Innate
Interferon-beta / immunology,  metabolism
NF-kappa B / immunology,  metabolism
Signal Transduction*
Chemical
Reg. No./Substance:
0/NF-kappa B; 77238-31-4/Interferon-beta; 80295-33-6/Complement C1q; EC 3.6.1.-/DDX58 protein, human; EC 3.6.1.-/DEAD-box RNA Helicases

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