Document Detail


A competitive enzyme-linked immunoassay for domoic acid determination in human body fluids.
MedLine Citation:
PMID:  7813986     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A polyclonal antiserum was raised in mice against domoic acid. Two of three immunogens consisted of domoic acid coupled to ovalbumin (OVA) and keyhole limpet haemocyanin at molar ratios of 47:1 and 44:1, respectively using a carbodiimide reaction. Titres of both antisera exceeded 1/35,000 against domoic acid coupled to the non-relevant carrier. Domoic acid was also conjugated to bovine serum albumin at a molar ratio of 30:1 using N-hydroxysuccinimidyl-4-azidobenzoate, a photoreactive compound. This immunogen, however, produced no measurable serum titres against domoic acid. The antiserum produced against the OVA conjugate displayed the highest affinity for free domoic acid in competitive enzyme-linked immunosorbent assay (ELISA). Furthermore, this antiserum preparation did not significantly cross-react with glutamic acid, aspartic acid, the structural analogue kainic acid, or the paralytic shellfish toxin, saxitoxin. The competitive ELISA was used to quantify domoic acid concentrations in human body fluids spiked with pure domoate. The lower limits of accurate domoic acid determinations in competitive ELISA were 0.2 micrograms/ml in urine, 0.25 micrograms/ml in plasma and 10 micrograms/ml in milk. It was concluded that the competitive ELISA described herein could be used to quantitate directly the concentration of domoic acid in the body fluids of individuals with amnesic shellfish poisoning.
Authors:
D S Smith; D D Kitts
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association     Volume:  32     ISSN:  0278-6915     ISO Abbreviation:  Food Chem. Toxicol.     Publication Date:  1994 Dec 
Date Detail:
Created Date:  1995-02-06     Completed Date:  1995-02-06     Revised Date:  2013-11-14    
Medline Journal Info:
Nlm Unique ID:  8207483     Medline TA:  Food Chem Toxicol     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  1147-54     Citation Subset:  IM    
Affiliation:
Department of Food Sciences, University of British Columbia, Vancouver, Canada.
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MeSH Terms
Descriptor/Qualifier:
Animals
Binding, Competitive
Enzyme-Linked Immunosorbent Assay*
Female
Foodborne Diseases / diagnosis
Hemocyanin / metabolism
Humans
Immune Sera / diagnostic use
Kainic Acid / analogs & derivatives*,  analysis,  metabolism,  poisoning
Male
Marine Toxins / analysis*,  metabolism,  poisoning
Mice
Mice, Inbred BALB C
Milk, Human / chemistry
Neurotoxins / analysis*,  metabolism,  poisoning
Ovalbumin / metabolism
Sensitivity and Specificity
Serum Albumin, Bovine / metabolism
Shellfish Poisoning
Chemical
Reg. No./Substance:
0/Immune Sera; 0/Marine Toxins; 0/Neurotoxins; 0/Serum Albumin, Bovine; 487-79-6/Kainic Acid; 9006-59-1/Ovalbumin; 9013-72-3/Hemocyanin; FV4Y0JO2CX/keyhole-limpet hemocyanin; M02525818H/domoic acid

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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