| A comparison of vasopressin and noradrenaline on oxygen uptake by perfused rat hindlimb, kidney, intestine and mesenteric arcade suggests that it is in part due to contractile work by blood vessels. | |
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MedLine Citation:
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PMID: 2276598 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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1. The rat hindlimb, kidney and intestine were each perfused in a nonrecirculating mode at 25 degrees C using an artificial perfusate (initial pressure 85 +/- 5 mmHg) and the effects of vasopressin and noradrenaline on oxygen uptake and perfusion pressure determined. 2. Both vasopressin (K0.5 = 0.1 nM) and noradrenaline (K0.5 = 2 nM) increased oxygen uptake as well as perfusion pressure by the perfused hindlimb; changes in oxygen uptake were closely matched by changes in pressure. The maximum increase in oxygen uptake was approx. 9 mumol/hr per g wet wt of hindlimb. 3. The perfused kidney also responded to vasopressin and noradrenaline with parallel increases in oxygen uptake and perfusion pressure for each agent. The largest increase in oxygen uptake was approx. 30 mumol/hr per g wet wt but this was not maximal. 4. Vasopressin increased oxygen uptake and pressure by the perfused intestine over the range 0.01-2 nM, but the changes in pressure only became significant at doses greater than 0.1 nM. 5. Noradrenaline inhibited oxygen uptake and increased perfusion pressure in a dose-dependent manner at pharmacological concentrations (greater than 30 nM) when shunting of perfusate may have contributed to unperfused regions. 6. A network of mesenteric blood vessels estimated to contain approx. 6% vascular tissue by weight, with the remainder white fat cells, lymphatics and connective tissue, was also perfused. 7. Vasopressin (K0.5 = 0.3 nM) and noradrenaline (K0.5 = 30 nM) each increased oxygen uptake and perfusion pressure in a dose-dependent manner.(ABSTRACT TRUNCATED AT 250 WORDS) |
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Authors:
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J M Ye; E Q Colquhoun; M G Clark |
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Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: General pharmacology Volume: 21 ISSN: 0306-3623 ISO Abbreviation: Gen. Pharmacol. Publication Date: 1990 |
Date Detail:
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Created Date: 1991-03-01 Completed Date: 1991-03-01 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 7602417 Medline TA: Gen Pharmacol Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 805-10 Citation Subset: IM |
Affiliation:
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Department of Biochemistry, University of Tasmania, Hobart, Australia. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Blood Pressure / drug effects Body Temperature / drug effects Hindlimb / drug effects, metabolism Intestines / drug effects, metabolism Kidney / drug effects, metabolism Male Mesentery / drug effects, metabolism Muscle Contraction / drug effects Muscle, Smooth, Vascular / drug effects* Norepinephrine / pharmacology* Oxygen Consumption / drug effects* Rats Rats, Inbred Strains Splanchnic Circulation / drug effects* Vasopressins / pharmacology* |
| Chemical | |
Reg. No./Substance:
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11000-17-2/Vasopressins; 51-41-2/Norepinephrine |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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