Document Detail


A comparison of various agarose preparations in a counter--immunoelectrophoresis (CIE) system for assaying urinary myoglobin.
MedLine Citation:
PMID:  833429     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Twenty-two samples of commercial agarose were utilized as the gel matrix in a counter-immunoelectrophoresis (CIE) for detection of urinary myoglobin. When different samples were tested under identical conditions, significant differences in the detectable levels of myoglobin by CIE could be traced to the quality of the agarose used. The quality of agarose preparation could be improved by repurification using polyethylene glycol precipitation. The necessity of using purified agarose when assaying for urinary myoglobin by CIE is stressed. This is particularly important when the test is used in the early detection of myocardial infarcts.
Authors:
H Hibrawi; F D Garrison; H J Smith
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Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Journal of immunological methods     Volume:  14     ISSN:  0022-1759     ISO Abbreviation:  J. Immunol. Methods     Publication Date:  1977  
Date Detail:
Created Date:  1977-03-15     Completed Date:  1977-03-15     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  1305440     Medline TA:  J Immunol Methods     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  59-63     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Counterimmunoelectrophoresis*
Humans
Immunoelectrophoresis*
Myoglobinuria / diagnosis*
Polysaccharides*
Sepharose*
Chemical
Reg. No./Substance:
0/Polysaccharides; 9012-36-6/Sepharose

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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