Document Detail


A comparison of two cloned mouse beta-globin genes and their surrounding and intervening sequences.
MedLine Citation:
PMID:  566620     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The BALC/c mouse has two nonallelic beta-globin genes that appear to reside on two different Eco R1 fragments of genomic DNA. We have already cloned one of these fragments and shown that the gene encoded within it is interrupted by at least one large intervening sequence of DNA. We have now cloned and characterized the second beta-globin gene-containing fragment. The coding sequence of its gene is also interrupted by an intervening sequence of DNA that occurs in about the same position, relative to the coding sequence, as does the first. Because some shared features of the structure of these two genes might be responsible for their coordinate expression and the elimination of their intervening sequences, we have compared their surrounding, coding and intervening sequences by restriction endonuclease analysis and by visualization of the heteroduplex structures formed between them. Of the 7000 bp of sequence compared in this way, we find only a few hundred base pairs of homology in addition to the coding sequence. These shared sequences flank the coding sequence and appear to include only those portions of the intervening sequence immediately adjacent to the interrupted structural gene.
Authors:
D C Tiemeier; S M Tilghman; F I Polsky; J G Seidman; A Leder; M H Edgell; P Leder
Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Cell     Volume:  14     ISSN:  0092-8674     ISO Abbreviation:  Cell     Publication Date:  1978 Jun 
Date Detail:
Created Date:  1978-09-15     Completed Date:  1978-09-15     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0413066     Medline TA:  Cell     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  237-45     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Base Sequence
Chromosome Mapping
DNA / genetics*
DNA Restriction Enzymes
Genes*
Globins / biosynthesis*
Mice
Mice, Inbred BALB C
Microscopy, Electron
Chemical
Reg. No./Substance:
9004-22-2/Globins; 9007-49-2/DNA; EC 3.1.21.-/DNA Restriction Enzymes

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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