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A comparison of transcriptional linkage of tRNA cistrons in yeast and E. coli by the ultraviolet light technique.
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MedLine Citation:
PMID:  333396     Owner:  NLM     Status:  MEDLINE    
The ultraviolet light mapping technique was employed to determine the lengths of tRNA cistrons in yeast. The applicability of the method was first tested in the E. coli system, in which the mapping positions for some tRNA cistrons and the ribosomal 5S RNA genes as well as the existence of multimeric transcription units for tRNAs are known. Rates of the synthesis of the tRNAs and small rRNAs after irradiation with various doses of UV light were determined by pulse labeling and quantitation of the RNA species after twodimensional gel electrophoreses. The small ribosomal RNAs served for internal calibration in the estimtion of the target sizes. Our results suggest that--in contrast to the prokaryotic system--in yeast the majority of the tRNA genes are not linked into transcriptional units.
H Feldman
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Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Nucleic acids research     Volume:  4     ISSN:  0305-1048     ISO Abbreviation:  Nucleic Acids Res.     Publication Date:  1977 Aug 
Date Detail:
Created Date:  1977-11-30     Completed Date:  1977-11-30     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  0411011     Medline TA:  Nucleic Acids Res     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  2831-41     Citation Subset:  IM    
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MeSH Terms
Dose-Response Relationship, Radiation
Escherichia coli / genetics*,  metabolism,  radiation effects
RNA, Transfer* / genetics,  radiation effects
Saccharomyces cerevisiae / metabolism,  radiation effects*
Species Specificity
Transcription, Genetic* / radiation effects*
Ultraviolet Rays*
Reg. No./Substance:
9014-25-9/RNA, Transfer

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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Journal Information
Journal ID (nlm-ta): Nucleic Acids Res
ISSN: 0305-1048
ISSN: 1362-4962
Article Information
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Print publication date: Month: 8 Year: 1977
Volume: 4 Issue: 8
First Page: 2831 Last Page: 2841
ID: 342612
PubMed Id: 333396

A comparison of transcriptional linkage of tRNA cistrons in yeast and E. coli by the ultraviolet light technique.
H Feldman

Article Categories:
  • Research Article

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