Document Detail


A comparison of three real-time PCR assays for the confirmation of Neisseria gonorrhoeae following detection of N. gonorrhoeae using Roche COBAS AMPLICOR.
MedLine Citation:
PMID:  18294244     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Three different real-time PCR assays were evaluated as confirmatory tests for Neisseria gonorrhoeae after initial screening using the COBAS AMPLICOR Chlamydia trachomatis and N. gonorrhoeae duplex assay. The target genes used for the confirmation were the gyr, cppB and 16S rRNA genes. Analytical specificity was determined by testing 60 strains belonging to different bacterial species and/or serogroups. The primers chosen from the 16S rRNA gene for confirmation of N. gonorrhoeae were highly specific, showed no cross-reactivity with other bacteria included in the study, and had an analytical sensitivity of 1 CFU. Of 192 clinical specimens that were positive for N. gonorrhoeae according to the COBAS AMPLICOR assay, 42 were confirmed as positive using the 16S rRNA gene target, 26 were confirmed using the cppB target, and 30 were confirmed using the gyr target. It was concluded that the real-time PCR assay targeting the 16S rRNA gene is a useful confirmatory assay to complement the COBAS AMPLICOR screening test for N. gonorrhoeae.
Authors:
L Chui; T Chiu; J Kakulphimp; G J Tyrrell
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Publication Detail:
Type:  Comparative Study; Journal Article     Date:  2008-02-22
Journal Detail:
Title:  Clinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases     Volume:  14     ISSN:  1198-743X     ISO Abbreviation:  Clin. Microbiol. Infect.     Publication Date:  2008 May 
Date Detail:
Created Date:  2008-04-10     Completed Date:  2008-06-18     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9516420     Medline TA:  Clin Microbiol Infect     Country:  France    
Other Details:
Languages:  eng     Pagination:  473-9     Citation Subset:  IM    
Affiliation:
Department of Laboratory Medicine and Pathology, University of Alberta, Edmonton, AB, Canada. l.chui@provlab.ab.ca
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MeSH Terms
Descriptor/Qualifier:
Bacterial Outer Membrane Proteins / analysis
DNA Gyrase / analysis
Female
Gonorrhea / diagnosis*
Humans
Male
Neisseria gonorrhoeae / isolation & purification*
Polymerase Chain Reaction / methods*
RNA, Bacterial / analysis
RNA, Ribosomal, 16S / analysis
Chemical
Reg. No./Substance:
0/Bacterial Outer Membrane Proteins; 0/RNA, Bacterial; 0/RNA, Ribosomal, 16S; 0/cppB protein, Neisseria gonorrhoeae; EC 5.99.1.-/DNA Gyrase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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