Document Detail


A comparison of three rapid and accurate bioluminescent antibiotic susceptibility tests.
MedLine Citation:
PMID:  19861166     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
INTRODUCTION: Rapid determination of microorganisms' antibiotic susceptibility remains a great challenge for the clinical diagnosis of infectious diseases. In this study, we compare three rapid bioluminescent antibiotic susceptibility tests with the conventional normalised microdilution method. METHODS: The susceptibility of two bacterial and two fungal strains to a 6h incubation with one of eight standard antibiotics was investigated by the ATP, AK and QNO bioluminescent methods. RESULTS: Three rapid and accurate antibiotic susceptibility testing were investigated performing ATP, AK and QNO bioluminescent assays. All the results were in accordance with those obtained using a standard microdilution assay. All these methods were sensitive and faster than the previously well-known ones. DISCUSSION: The development of bioluminescent determination methods could lead to accurate and useful antibiotic susceptibility testing.
Authors:
Mickael Lafond; Nicolas Vidal; Yves Letourneux; Jean Michel Brunel
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Publication Detail:
Type:  Comparative Study; Journal Article     Date:  2009-10-25
Journal Detail:
Title:  Journal of pharmacological and toxicological methods     Volume:  61     ISSN:  1873-488X     ISO Abbreviation:  J Pharmacol Toxicol Methods     Publication Date:    2010 Jan-Feb
Date Detail:
Created Date:  2010-02-03     Completed Date:  2010-04-20     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9206091     Medline TA:  J Pharmacol Toxicol Methods     Country:  United States    
Other Details:
Languages:  eng     Pagination:  16-9     Citation Subset:  IM    
Copyright Information:
Copyright 2009 Elsevier Inc. All rights reserved.
Affiliation:
UPCAM iSm2, Case 342, Universit? Paul C?zanne, Av. Escadrille Normandie Ni?men, 13397 Marseille cedex 13, France.
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MeSH Terms
Descriptor/Qualifier:
Adenylate Kinase / metabolism
Anti-Bacterial Agents / pharmacology*
Antifungal Agents / pharmacology*
Candida albicans / drug effects
Escherichia coli / drug effects
Luminescent Agents / metabolism
Microbial Sensitivity Tests / methods*
NAD(P)H Dehydrogenase (Quinone) / metabolism
Reproducibility of Results
Saccharomyces cerevisiae / drug effects
Staphylococcus aureus / drug effects
Time Factors
Chemical
Reg. No./Substance:
0/Anti-Bacterial Agents; 0/Antifungal Agents; 0/Luminescent Agents; EC 1.6.5.2/NAD(P)H Dehydrogenase (Quinone); EC 2.7.4.3/Adenylate Kinase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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