Document Detail


A comparison of three methods for detecting the acrosome reaction in human spermatozoa.
MedLine Citation:
PMID:  8671320     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
This study was designed to compare three different fluorescent probes to assay the acrosome reaction in human spermatozoa: chlortetracycline (CTC), mannosylated bovine serum albumin (BSA) labelled with fluorescein (MAF), and quinacrine (QN). Normal human sperm ejaculates were washed and allowed to swim up for 30-60 min. Samples were examined under epifluorescence for the percentage of the acrosome reacted spermatozoa, as detected by the three probes. There was no significant differences between samples of fresh, uncapacitated spermatozoa evaluated with CTC, MAF or QN; all gave < 10% reacted. Following capacitation for 3 h, the percentage of spontaneously reacted spermatozoa was higher than in fresh spermatozoa; CTC and MAF gave the same percentage (12%), while QN indicated a higher percentage (18%) of reacted spermatozoa (P < 0.001). Following exposure to ionophore A23187 at 1 h, the percentage of acrosome reactions increased to a mean of 31% as detected with CTC or MAF; the mean percentage (45%) was significantly higher with QN (P < 0.0001). Further incubation up to 2 h with A23187 did not change these percentages. These results suggest that the QN probe detects the onset stage of the acrosome reaction, whereas the CTC and MAF probes detect the later stages in which the acrosomal cap is lost. Use of the two types of probe provides a means for finer resolution of the time course of the acrosome reaction in the human spermatozoa.
Authors:
A H Amin; J L Bailey; B T Storey; L Blasco; S Heyner
Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Human reproduction (Oxford, England)     Volume:  11     ISSN:  0268-1161     ISO Abbreviation:  Hum. Reprod.     Publication Date:  1996 Apr 
Date Detail:
Created Date:  1996-11-21     Completed Date:  1996-11-21     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  8701199     Medline TA:  Hum Reprod     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  741-5     Citation Subset:  IM    
Affiliation:
Department of Obstetrics and Gynecology, University of Pennsylvania Medical Center, Philadelphia 19104-6080, USA.
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MeSH Terms
Descriptor/Qualifier:
Acrosome / physiology*
Animals
Cattle
Female
Fluorescence
Fluorescent Dyes
Humans
Male
Sperm-Ovum Interactions*
Spermatozoa / physiology*
Grant Support
ID/Acronym/Agency:
HD-06274/HD/NICHD NIH HHS
Chemical
Reg. No./Substance:
0/Fluorescent Dyes

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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