Document Detail


A comparison of six different plating media used in the isolation of Salmonella.
MedLine Citation:
PMID:  7986679     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Seventeen Canadian Federal, Provincial and Public Health Laboratories took part in different phases of a comparative/collaborative study that evaluated rapid methods to the standard Health Protection Branch (HPB) method for the detection of Salmonella. A variety of commercial media were tested, including Brilliant Green Sulpha Agar, Bismuth Sulphite Agar, Hektoen Enteric Agar, Xylose Lysine Deoxycholate Agar, EF-18 Agar and Rambach Agar. Each laboratory compared up to six of these different plating media. Plating of 123 salmonellae cultures and 28 artificially-inoculated foods showed the recovery of Salmonella spp. on the six plating media to be within one log. Therefore, quantitative testing of the media showed them to be comparable in the recovery of salmonellae. Qualitative testing of the six media during the comparative/collaborative study of various methods showed that EF-18 Agar recovered the greatest number of isolates. Hektoen Enteric Agar ranked second, with the other agars being comparable in their recovery of Salmonella spp. Problems with the various media are summarized. Based on our results and those of other researchers, it is recommended that Bismuth Sulphite Agar be compulsory and that at least one other agar be used for newly developed cultural procedures.
Authors:
D W Warburton; B Bowen; A Konkle; C Crawford; S Durzi; R Foster; C Fox; L Gour; G Krohn; P LaCasse
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Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  International journal of food microbiology     Volume:  22     ISSN:  0168-1605     ISO Abbreviation:  Int. J. Food Microbiol.     Publication Date:  1994 Jun 
Date Detail:
Created Date:  1995-01-12     Completed Date:  1995-01-12     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  8412849     Medline TA:  Int J Food Microbiol     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  277-89     Citation Subset:  IM    
Affiliation:
Evaluation Division, Bureau of Microbial Hazards, HPB Health and Welfare Canada, Ottawa.
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MeSH Terms
Descriptor/Qualifier:
Bacteriological Techniques*
Canada
Culture Media*
Evaluation Studies as Topic
False Positive Reactions
Food Microbiology*
Food Technology
Government Agencies
Laboratories
Questionnaires
Salmonella / isolation & purification*
Chemical
Reg. No./Substance:
0/Culture Media

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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