Document Detail


A comparison of the properties of the binary and ternary complexes formed by calmodulin and troponin C with two regulatory peptides of phosphorylase kinase.
MedLine Citation:
PMID:  8672716     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The regulatory peptides Phk13 (301-327) and a modified form of Phk5 (342-367) from the gamma-subunit of glycogen phosphorylase kinase form binary and ternary complexes with both calmodulin and the related muscle protein troponin C. Neither peptide appears to affect to a major extent a fluorescent probe linked to Cys-27 of wheat germ calmodulin. Phk13, but not Phk5, significantly modifies the properties of a probe joined to Cys-98 of troponin C. A comparison by means of radiationless energy transfer of the average separations of Trp-16 of Phk5 from specific groups in the N- and C-terminal halves of calmodulin and troponin C indicate significant changes upon going from the 1:1 binary complex to the 1:1:1 ternary complex with Phk13. A comparison of the effects of addition of Phk13 to calmodulin, troponin C, and their binary complexes with Phk5 suggests that the conformation of Phk13 is similar in the binary and ternary complexes.
Authors:
R F Steiner; D Juminaga; S Albaugh; H Washington
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Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Biophysical chemistry     Volume:  59     ISSN:  0301-4622     ISO Abbreviation:  Biophys. Chem.     Publication Date:  1996 Apr 
Date Detail:
Created Date:  1996-08-13     Completed Date:  1996-08-13     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  0403171     Medline TA:  Biophys Chem     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  277-88     Citation Subset:  IM    
Affiliation:
Department of Chemistry and Biochemistry, University of Maryland, Baltimore 21228, USA.
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Sequence
Animals
Calmodulin / chemistry*
Cattle
Circular Dichroism
Molecular Sequence Data
Peptides / chemistry*
Phosphorylase Kinase / chemistry*
Rabbits
Spectrometry, Fluorescence
Troponin / chemistry*
Troponin C
Trypsin
Tryptophan / chemistry
Chemical
Reg. No./Substance:
0/Calmodulin; 0/Peptides; 0/Troponin; 0/Troponin C; 73-22-3/Tryptophan; EC 2.7.1.19/Phosphorylase Kinase; EC 3.4.21.4/Trypsin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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