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A comparison of the promoter strengths of two eukaryotic genes in vitro reveals a region of DNA that can influence the rate of transcription in cis over long distances.
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MedLine Citation:
PMID:  6310493     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We have compared the strength of a trout protamine gene promoter with that of the mouse beta major-globin gene by analysing the relative levels of run-off transcripts produced in a single mammalian in vitro transcription reaction. When the promoters are introduced on separate recombinant plasmids, the protamine transcripts are synthesised with much greater efficiency than those originating from the globin cap site. This enhanced transcription of the protamine gene is again observed when the promoters are applied as separate DNA fragments derived from the same recombinant plasmid. However, when the promoters are linked on a DNA fragment that includes 7 kb of DNA separating the initiation sites, then there is a marked reduction in the protamine signal relative to the globin. Deletion of a region of this fragment that contains the sequences flanking the globin gene at positions -335 to -1400 restores the enhanced protamine gene expression to the levels observed when the promoters are carried on separate DNA fragments.
Authors:
S P Gregory; P H Butterworth
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Nucleic acids research     Volume:  11     ISSN:  0305-1048     ISO Abbreviation:  Nucleic Acids Res.     Publication Date:  1983 Aug 
Date Detail:
Created Date:  1983-10-08     Completed Date:  1983-10-08     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  0411011     Medline TA:  Nucleic Acids Res     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  5317-26     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Base Sequence
DNA Restriction Enzymes
DNA, Recombinant / metabolism
Genes*
Globins / genetics*
Mice
Nucleic Acid Hybridization
Operon*
Protamines / genetics*
Species Specificity
Transcription, Genetic*
Trout
Chemical
Reg. No./Substance:
0/DNA, Recombinant; 0/Protamines; 9004-22-2/Globins; EC 3.1.21.-/DNA Restriction Enzymes
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Full Text
Journal Information
Journal ID (nlm-ta): Nucleic Acids Res
ISSN: 0305-1048
ISSN: 1362-4962
Article Information
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Print publication date: Day: 25 Month: 8 Year: 1983
Volume: 11 Issue: 16
First Page: 5317 Last Page: 5326
ID: 326280
PubMed Id: 6310493

A comparison of the promoter strengths of two eukaryotic genes in vitro reveals a region of DNA that can influence the rate of transcription in cis over long distances.
S P Gregory
P H Butterworth



Article Categories:
  • Research Article


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