| A comparison of the proliferative capacity and ultrastructure of proliferative cells from the cochleae of newborn rats of different ages. | |
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MedLine Citation:
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PMID: 19963281 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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OBJECTIVE: Recent reports have shown that multipotent stem cells/progenitor cells that are capable of proliferation and regeneration are present in mammalian cochleae. However, progenitor cells have not been isolated from the adult cochlea. We examined the proliferative potential of cells derived from neonatal rats of various ages. The determination of the differences between the proliferative cells from rats of different ages may provide clues to the mechanisms controlling the destiny of these cells. METHODS: Proliferative cells were isolated from the cochleae of 1-, 7-, and 14-day-old rats, and the proliferative capacity and ultrastructure of the cells from each age group were assessed using flow cytometry and transmission electron microscopy, respectively. RESULTS: During the first two postnatal weeks, the number of proliferative cells gradually fell to zero. This decrease occurred in parallel with the impairment of the proliferative capacity of the cells and the accumulation of proliferative cells in G0/G1. In addition, some of the cells exited the cell cycle by means of gradual maturity and apoptosis. CONCLUSIONS: Our study suggests that cochlear proliferative cells are remnants of the progenitor cells that originally gave rise to the sensory epithelium. The disappearance of the cochlear proliferative cells in adult mammalian cochleae may result from their differentiation and/or apoptosis. |
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Authors:
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Cuiping Zhong; Yu Han; Jianhua Qiu; Lianjun Lu; Yang Chen; Jun Chen; Renyi Hei; Wenjuan Mi |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2009-12-05 |
Journal Detail:
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Title: International journal of pediatric otorhinolaryngology Volume: 74 ISSN: 1872-8464 ISO Abbreviation: Int. J. Pediatr. Otorhinolaryngol. Publication Date: 2010 Feb |
Date Detail:
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Created Date: 2010-02-01 Completed Date: 2010-04-26 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8003603 Medline TA: Int J Pediatr Otorhinolaryngol Country: Ireland |
Other Details:
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Languages: eng Pagination: 192-7 Citation Subset: IM |
Copyright Information:
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Crown Copyright (c) 2009. Published by Elsevier Ireland Ltd. All rights reserved. |
Affiliation:
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Department of Otolaryngology, Xijing Hospital, Fourth Military Medical University, Changlexilu 15, Xi'an 710032, Shanxi Province, China. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Animals, Newborn Apoptosis / physiology Cell Proliferation Cochlea / metabolism, ultrastructure* Epithelium / ultrastructure Flow Cytometry Humans Intermediate Filament Proteins / metabolism Nerve Tissue Proteins / metabolism Rats Rats, Sprague-Dawley Spiral Ganglion / ultrastructure |
| Chemical | |
Reg. No./Substance:
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0/Intermediate Filament Proteins; 0/Nerve Tissue Proteins; 0/nestin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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