Document Detail

A comparison of multiplex suspension array large-panel kits for profiling cytokines and chemokines in rheumatoid arthritis patients.
MedLine Citation:
PMID:  18823005     Owner:  NLM     Status:  MEDLINE    
BACKGROUND: Multiplex analysis allows measurements of a large number of analytes simultaneously in each sample. On the basis of the Luminex multiplex technology (xMAP), kits for measuring multiple cytokines and chemokines (immunomodulators) are commercially available and are useful in investigations on inflammatory diseases. This study evaluated four multiplex kits (Bio-Plex, LINCOplex, Fluorokine, and Beadlyte) that contained 27, 29, 20, and 22 analytes each, respectively, for the analysis of immunomodulators in plasma of patients with rheumatoid arthritis (RA) who underwent treatment with antibody against CD20 (rituximab), a B-cell reductive therapy. METHODS: Multiplex kits were tested on serial plasma samples obtained from six RA patients at baseline and multiple time points (3, 6, and 9 months) post-treatment with rituximab. The RA patients included in this study had previously failed therapy with disease modifying anti-arthritis drugs (DMARD) and treatment with anti-TNFalpha antibody (infliximab). RESULTS: Computer modeling and hierarchical cluster analysis of the multiplex data allowed a comparison of the performance of multiplex assay kits and revealed profiles of immunomodulators in the RA patients. CONCLUSIONS: In plasma of RA patients who appeared to have benefited from the rituximab treatment, the profile of significantly elevated immunomodulators by at least two of the three kits (BioPlex, LINCOplex, Beadlyte) is as follows: IL-12p70, Eotaxin, IL-4, TNFalpha, Il-9, IL-1beta, IFNgamma, IL-10, IL-6, and IL-13. Immunomodulator profiling by multiplex analysis may provide useful plasma biomarkers for monitoring response to B-cell reductive therapy in RA patients.
Imran H Khan; V V Krishnan; Melanie Ziman; Kim Janatpour; Ted Wun; Paul A Luciw; Joseph Tuscano
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Cytometry. Part B, Clinical cytometry     Volume:  76     ISSN:  1552-4957     ISO Abbreviation:  Cytometry B Clin Cytom     Publication Date:  2009 May 
Date Detail:
Created Date:  2009-04-14     Completed Date:  2009-07-20     Revised Date:  2009-12-11    
Medline Journal Info:
Nlm Unique ID:  101235690     Medline TA:  Cytometry B Clin Cytom     Country:  United States    
Other Details:
Languages:  eng     Pagination:  159-68     Citation Subset:  IM    
Copyright Information:
(c) 2008 Clinical Cytometry Society.
Center for Comparative Medicine, University of California, Davis, CA 95616, USA.
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MeSH Terms
Antibodies, Monoclonal / administration & dosage
Arthritis, Rheumatoid / blood*,  drug therapy
Chemistry, Clinical / methods*
Chemokines / blood*
Cluster Analysis
Computer Simulation
Cytokines / blood*
Infusions, Intravenous
Middle Aged
Reagent Kits, Diagnostic*
Grant Support
Reg. No./Substance:
0/Antibodies, Monoclonal; 0/Chemokines; 0/Cytokines; 0/Reagent Kits, Diagnostic; 0/rituximab

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