Document Detail


The comparison of methods to identify the presence of fibrocytes in formalin-fixed, paraffin-embedded archival cardiac tissue with coronary heart disease.
MedLine Citation:
PMID:  25155375     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
The purpose of this study was to find the optimal technical approach to identify the presence of fibrocytes in formalin-fixed, paraffin-embedded archival cardiac tissue with CHD (coronary heart disease). Using the coexpression markers CD45 and αSMA, the presence of fibrocytes was examined by three different methods, including double immunohistochemistry staining, combination labeling of immunohistochemistry and immunofluorescence and double immunofluorescence labeling. Double immunohistochemistry staining was very difficult to identify the CD45(+)/αSMA(+) fibrocytes. Although combination staining of immunohistochemistry and immunofluorescence has made it possible to evaluate the co-localization of CD45 and αSMA in the fibrocytes, this method was prone to produce many false positive cells. In contrast, CD45(+)/αSMA(+) fibrocytes could be clearly recognized by double immunofluorescence labeling. In conclusion, double immunofluorescence labeling is the optimal technical approach to identify the presence of fibrocytes in routinely processed cardiac tissue with CHD.
Authors:
Pu-Ping Lei; Qun Shuai; Shang-Wen Wang; Si-Ming Tao; Yong-Qiang Qu; Dian-Hua Wang
Related Documents :
3711535 - Transient myocardial dysfunction during pharmacologic vasodilation as an index of reduc...
6154835 - Hemodynamic and cardiac effects of clonidine.
18431525 - No and pgi(2) in coronary endothelial dysfunction in transgenic mice with dilated cardi...
21983305 - Titanium-nitride-oxide-coated titan-2 bioactive coronary stent: a new breakthrough in i...
24513915 - Localizing the critical isthmus of post-infarct ventricular tachycardia: the value of p...
3581835 - Bedside intracoronary thrombolysis in the coronary care unit.
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2014-7-22
Journal Detail:
Title:  Pathology, research and practice     Volume:  -     ISSN:  1618-0631     ISO Abbreviation:  Pathol. Res. Pract.     Publication Date:  2014 Jul 
Date Detail:
Created Date:  2014-8-26     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7806109     Medline TA:  Pathol Res Pract     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2014 Elsevier GmbH. All rights reserved.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  The use of the sterically demanding IPr* and related ligands in catalysis.
Next Document:  Fascin and EMMPRIN expression in primary mucinous tumors of ovary: A tissue microarray study.