| A comparison of methods for blocking staining of nucleic acids. | |
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MedLine Citation:
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PMID: 80907 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Using rat spinal cord as test material 4 methods for blocking staining of nucleic acids with simple basic dyes have been compared. Two of these methods--a perchloric acid procedure for tissue blocks and a Ba(OH)2 technic for sections--block staining of RNA. The other two-immersion of sections in solutions of dichlorodiammine Pt II or in 10% zirconyl chloride-blocked staining of both RNA and DNA. None of these methods hydrolyze DNA sufficiently to produce a positive Feulgen and no loss of protein could be demonstrated. With the three metal containing procedures some metal could be demonstrated in the sections. This deposition was primarily in nucleic acid containing structures and was greatest and most widespread with the zirconyl chloride. The latter method altered the color of all hematoxylin technics to a deep red. The separation of nuclear stains on the basis of their bonding characteristics into three groups (Lillie et al. 1976) was confirmed. |
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Authors:
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G Clark; S Meischen |
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Publication Detail:
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Type: Comparative Study; Journal Article |
Journal Detail:
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Title: Acta histochemica Volume: 61 ISSN: 0065-1281 ISO Abbreviation: Acta Histochem. Publication Date: 1978 |
Date Detail:
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Created Date: 1978-11-18 Completed Date: 1978-11-18 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0370320 Medline TA: Acta Histochem Country: GERMANY, EAST |
Other Details:
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Languages: eng Pagination: 192-6 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cell Nucleolus / metabolism Coloring Agents Hematoxylin Histological Techniques* Hydroxides Neuroglia / metabolism Nucleic Acids / metabolism* Perchloric Acid Rats Spinal Cord / metabolism Staining and Labeling |
| Chemical | |
Reg. No./Substance:
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0/Coloring Agents; 0/Hydroxides; 0/Nucleic Acids; 517-28-2/Hematoxylin; 7601-90-3/Perchloric Acid |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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