| A comparison of membrane properties and composition between cell lines selected and transfected for multi-drug resistance. | |
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MedLine Citation:
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PMID: 1358166 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Cell lines selected (CHRC5) and transfected (LR-73-1A) for multi-drug resistance have total lipid compositions which are indistinguishable between resistant and parental cells. Lipid composition was evaluated by 1H NMR and the total fatty acid content by GLC. No change in surface hydrophobicity, as measured with the fluorescent probe dansyl-PE, was observed as a result of transfection of CHO cells with the mdr1 gene. However, the selected cell line, CHRC5, showed a decreased surface hydrophobicity. This decreased surface hydrophobicity was indicated by an 8 nm increase in the fluorescence emission of dansyl-PE in the CHRC5 cell line compared with the AB1. Both resistant cell lines showed an increase in the polarisation of the fluorescent probe, TMA-DPH in the plasma membranes corresponding to a 14% and a 24% change in fluorescence polarisation for the selected and transfected cell lines, respectively. This is indicative of reduced mobility of the acyl chains in the resistant cell lines. Both the CHRC5 and the transfected cell lines showed almost a 2-fold increase in the initial rate of membrane cycling. The membrane cycling could be inhibited by a known bilayer stabiliser, the N-carbobenzoxy-D-Phe-L-Phe-Gly. These results indicate that the properties of the plasma membrane from resistant cells are altered compared with their parental cell line. |
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Authors:
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R Callaghan; L C van Gorkom; R M Epand |
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Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: British journal of cancer Volume: 66 ISSN: 0007-0920 ISO Abbreviation: Br. J. Cancer Publication Date: 1992 Nov |
Date Detail:
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Created Date: 1992-12-15 Completed Date: 1992-12-15 Revised Date: 2010-09-07 |
Medline Journal Info:
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Nlm Unique ID: 0370635 Medline TA: Br J Cancer Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 781-6 Citation Subset: IM |
Affiliation:
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Department of Biochemistry, McMaster University Health Sciences Centre, Hamilton, Ontario, Canada. |
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals CHO Cells / physiology, ultrastructure Cell Membrane / chemistry, physiology* Chemistry, Physical Chromatography, Gas / methods Cricetinae Dansyl Compounds Diphenylhexatriene Drug Resistance / genetics, physiology* Fatty Acids / analysis, physiology Fluorescence Polarization / methods Magnetic Resonance Spectroscopy / methods Membrane Glycoproteins / genetics, physiology Membrane Lipids / analysis, physiology* P-Glycoprotein Phosphatidylethanolamines Physicochemical Phenomena Quaternary Ammonium Compounds Transfection |
| Chemical | |
Reg. No./Substance:
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0/Dansyl Compounds; 0/Fatty Acids; 0/Membrane Glycoproteins; 0/Membrane Lipids; 0/P-Glycoprotein; 0/Phosphatidylethanolamines; 0/Quaternary Ammonium Compounds; 1720-32-7/Diphenylhexatriene; 37219-74-2/dansyl phosphatidylethanolamine; 51-92-3/tetramethylammonium |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
| Full Text | |
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Journal Information Journal ID (nlm-ta): Br J Cancer ISSN: 0007-0920 ISSN: 1532-1827 |
Article Information Download PDF  Print publication date: Month: 11 Year: 1992 Volume: 66 Issue: 5 First Page: 781 Last Page: 786 ID: 1977978 PubMed Id: 1358166 |
| A comparison of membrane properties and composition between cell lines selected and transfected for multi-drug resistance. | |
| R. Callaghan | |
| L. C. van Gorkom | |
| R. M. Epand | |
| Department of Biochemistry, McMaster University Health Sciences Centre, Hamilton, Ontario, Canada. |
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