Document Detail

A comparison of the efficiency of human embryo intestine, Hep 2 cells, and human embryo kidney cells for the primary isolation of ophthalmic viruses.
MedLine Citation:
PMID:  6271166     Owner:  NLM     Status:  MEDLINE    
A comparison has been made of the efficiency of 3 cell systems, human embryo kidney (HEK), Hep 2, and a continuous line of human embryo intestine (HEI), for the isolation of ophthalmic viruses. A total of 40 herpes simplex, 51 adenoviruses, and 2 vaccinia viruses were isolated from 323 specimens. HEK cells were found to be the optimal system, 85 out of 93 (91%) of the viruses being detected in these cells alone. However, HEK cells are difficult to obtain, and therefore the use of a combination of the continuous cell lines HEI and Hep 2 is recommended as an alternative. 89% of the viruses were detected by this combination. The use of either HEI or Hep 2 cells alone was unsatisfactory.
W D Cubitt; D A McSwiggan; U Thaker; S Darougar
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The British journal of ophthalmology     Volume:  65     ISSN:  0007-1161     ISO Abbreviation:  Br J Ophthalmol     Publication Date:  1981 Aug 
Date Detail:
Created Date:  1982-01-20     Completed Date:  1982-01-20     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  0421041     Medline TA:  Br J Ophthalmol     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  546-8     Citation Subset:  IM    
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MeSH Terms
Adenoviruses, Human / isolation & purification
Cell Line
Conjunctiva / microbiology*
Conjunctivitis / microbiology
DNA Viruses / isolation & purification*
Keratoconjunctivitis / microbiology
Simplexvirus / isolation & purification
Virus Cultivation / methods*

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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