Document Detail


A comparison of the effects of kaempferol and quercetin on cytokine-induced pro-inflammatory status of cultured human endothelial cells.
MedLine Citation:
PMID:  18394220     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We investigated the effects of the flavonols kaempferol and quercetin on the expression of vascular cell adhesion molecule-1 (VCAM-1), intercellular adhesion molecule-1 (ICAM-1), endothelial cell selectin (E-selectin), inducible NO synthase (iNOS) and cyclo-oxygenase-2 (COX-2), and on the activation of the signalling molecules NF-kappaB and activator protein-1 (AP-1), induced by a cytokine mixture in cultured human umbilical vein endothelial cells. Inhibition of reactive oxygen and nitrogen species generation did not differ among both flavonols at 1 micromol/l but was significantly stronger for kaempferol at 5-50 micromol/l. Supplementation with increasing concentrations of kaempferol substantially attenuated the increase induced by the cytokine mixture in VCAM-1 (10-50 micromol/l), ICAM-1 (50 micromol/l) and E-selectin (5-50 micromol/l) expression. A significantly inhibitory effect of quercetin on VCAM-1 (10-50 micromol/l), ICAM-1 (50 micromol/l) and E-selectin (50 micromol/l) expression was also observed. Expression of adhesion molecules was always more strongly inhibited in kaempferol-treated than in quercetin-treated cells. The inhibitory effect on iNOS and COX-2 protein level was stronger for quercetin at 5-50 micromol/l. The effect of kaempferol on NF-kappaB and AP-1 binding activity was weaker at high concentrations (50 micromol/l) as compared with quercetin. The present study indicates that differences exist in the modulation of pro-inflammatory genes and in the blockade of NF-kappaB and AP-1 by kaempferol and quercetin. The minor structural differences between both flavonols determine differences in their anti-inflammatory properties and in their efficiency in inhibiting signalling molecules.
Authors:
Irene Crespo; María V García-Mediavilla; Belén Gutiérrez; Sonia Sánchez-Campos; María J Tuñón; Javier González-Gallego
Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-04-08
Journal Detail:
Title:  The British journal of nutrition     Volume:  100     ISSN:  1475-2662     ISO Abbreviation:  Br. J. Nutr.     Publication Date:  2008 Nov 
Date Detail:
Created Date:  2008-10-07     Completed Date:  2008-11-18     Revised Date:  2009-05-20    
Medline Journal Info:
Nlm Unique ID:  0372547     Medline TA:  Br J Nutr     Country:  England    
Other Details:
Languages:  eng     Pagination:  968-76     Citation Subset:  IM    
Affiliation:
Centro de Investigación Biomédica en Red de Enfermedades Hepáticas y Digestivas (CIBEREHD) and Institute of Biomedicine, University of León, León 24071, Spain.
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MeSH Terms
Descriptor/Qualifier:
Analysis of Variance
Anti-Inflammatory Agents / pharmacology*
Biological Markers / analysis
Cells, Cultured
Cyclooxygenase 2 / analysis
Cytokines / pharmacology*
E-Selectin / analysis
Electrophoretic Mobility Shift Assay
Endothelial Cells / drug effects*,  immunology
Humans
Intercellular Adhesion Molecule-1 / analysis
Kaempferols / pharmacology*
NF-kappa B / analysis
Nitric Oxide Synthase Type II / analysis
Quercetin / pharmacology*
Transcription Factor AP-1 / analysis
Umbilical Veins
Vascular Cell Adhesion Molecule-1 / analysis
Chemical
Reg. No./Substance:
0/Anti-Inflammatory Agents; 0/Biological Markers; 0/Cytokines; 0/E-Selectin; 0/Kaempferols; 0/NF-kappa B; 0/Transcription Factor AP-1; 0/Vascular Cell Adhesion Molecule-1; 117-39-5/Quercetin; 126547-89-5/Intercellular Adhesion Molecule-1; 520-18-3/kaempferol; EC 1.14.13.39/Nitric Oxide Synthase Type II; EC 1.14.99.1/Cyclooxygenase 2

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