Document Detail

A comparison of the cytotoxic activity of eosinophils and other cells by 51 chromium release and time lapse microcinematography.
MedLine Citation:
PMID:  721138     Owner:  NLM     Status:  MEDLINE    
Antibody dependent cytotoxicity of chicken erythrocytes by purified rat eosinophils, neutrophils, macrophages and K cells has been compared by 51Cr release and time lapse microcinematography. Techniques have been developed for purifying these effector cell types. Both eosinophils and neutrophils cause rapid release of 51Cr from erythrocytes. Time lapse observations indicated that this was the result of phagocytosis. Eosinophils show rapid membrane movement and repeatedly engulf and regurgitate the erythrocytes. On the other hand, neutrophils become quiescent after phagocytosing erythrocytes, and remain quiescent until the remains of the cell are expelled. Neutrophils presumably have a mechanism for the release of soluble material, as 51Cr is released rapidly. Macrophages show a similar quiescence after phagocytosis, but in these cells there is apparently no rapid mechanism to expel material, as there is no significant 51Cr release over 20 h. K cells appear to damage chicken erythrocytes more slowly than they destroy tumour cells. Mast cells cause antibody-independent cytotoxicity which can be attributed to the release of toxic materials. None of these effector cells produced the type of lysis seen with antibody and complement.
C J Sanderson; J A Thomas
Related Documents :
16662488 - Relationship between phospholipid breakdown and freezing injury in a cell wall-less mut...
7620078 - Dimethyl sulphoxide: a review of its applications in cell biology.
20645048 - Cryopreservation of human hepatocytes for clinical use.
1401938 - A rapid and sensitive cellular enzyme-linked immunoabsorbent assay (celisa) for the det...
15334588 - 31p mas-nmr of human erythrocytes: independence of cell volume from angular velocity.
9551858 - Cell-cell signaling during neurogenesis: some answers and many questions.
Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Immunology     Volume:  34     ISSN:  0019-2805     ISO Abbreviation:  Immunology     Publication Date:  1978 Apr 
Date Detail:
Created Date:  1979-02-26     Completed Date:  1979-02-26     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  0374672     Medline TA:  Immunology     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  771-80     Citation Subset:  IM    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Antibody-Dependent Cell Cytotoxicity*
Cell Separation
Chromium Radioisotopes
Eosinophils / immunology*
Killer Cells, Natural / immunology
Macrophages / immunology
Mast Cells / immunology
Motion Pictures as Topic
Neutrophils / immunology
Reg. No./Substance:
0/Chromium Radioisotopes

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Induction of contact sensitivity. Selective induction of delayed hypersensitivity by the injection o...
Next Document:  Migration of human lymphocytes. I. A model using the mouse as host.