| A comparison of crypt-cell proliferation in rat colonic mucosa in vivo and in vitro. | |
| | |
MedLine Citation:
|
PMID: 3693105 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
The successful development of a long-term organ culture system has made it possible to perform experiments on rat colonic mucosa in vitro. However, the effect of trauma or the withdrawal of trophic influences in culture may result in the disturbance of proliferation within the tissue. In this paper we describe an investigation designed to characterise the culture system by a comparison of the proliferative parameters in vitro with those in vivo. Stathmokinetic experiments were performed in vivo and in vitro to estimate cell birth rate. Mitotic indices were also calculated. The in vivo birth rate (7.8 +/- 0.8 cells/1000 cells/hour) and the in vitro birth rate for the whole explant (7.7 +/- 0.5 cells/1000 cells/hour) were found to be similar. A study of crypts in the centre and at the edge of the cultured explants, however, indicated that proliferation at the two sites differed markedly, the birth rate at the edge (7.5 +/- 0.9 cells/1000 cells/hour) being approximately twice that at the centre (3.2 +/- 0.9 cells/1000 cells/hour). Provided that this topological difference in proliferation within the explant is recognised the in vitro model, in particular the basal level found at the centre, may still be regarded as a valid system for studying tissue responses to carcinogens and trophic factors. |
| | |
Authors:
|
K J Finney; P Ince; D R Appleton; J P Sunter; A J Watson |
Related Documents
:
|
10735125 - Effect of culture media on embryo development from prepubertal goat ivm-ivf oocytes. 1577945 - Production of normal mice from oocytes fertilized and developed without zonae pellucidae. 8931385 - Reduction of intracellular ph is not the mechanism for the synergistic interaction betw... 19533325 - Application of intra- and extracellular sugars and dimethylsulfoxide to human oocyte cr... 6230415 - Evidence for normal fibroblast cell membranes from individuals with huntington's diseas... 22840445 - Lethal effect of streptomyces citreofluorescens against larvae of malaria, filaria and ... |
Publication Detail:
|
Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: Journal of anatomy Volume: 149 ISSN: 0021-8782 ISO Abbreviation: J. Anat. Publication Date: 1986 Dec |
Date Detail:
|
Created Date: 1988-02-16 Completed Date: 1988-02-16 Revised Date: 2009-11-18 |
Medline Journal Info:
|
Nlm Unique ID: 0137162 Medline TA: J Anat Country: ENGLAND |
Other Details:
|
Languages: eng Pagination: 177-88 Citation Subset: IM |
Affiliation:
|
Department of Pathology, University of Newcastle upon Tyne. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Cell Division Colon / cytology* Intestinal Mucosa / cytology* Male Mitotic Index Organ Culture Techniques Rats Rats, Inbred Strains |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Motor innervation of rodent diaphragm.
Next Document: The number and distribution of lymphoid follicles in the human large intestine.