| A comparison of cell cycle-related changes in postmitotic and quiescent AF8 cells as measured by cytofluorometry after acridine orange staining. | |
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MedLine Citation:
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PMID: 667846 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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AF8 cells were collected by mitotic detachment or made quiescent by serum restriction. Replated mitotic cells or serum-stimulated quiescent cells were then compared by flow cytofluorometry, when the use of acridine orange staining. Red fluorescence intensity (F greater than 600) was the same in quiescent cells and in cells immediately after mitosis. However, F greater than 600 increased very rapidly in postmitotic cells, while there was a delay in serum-stimulated quiescent cells. F greater than 600 reached a peak at 4 hr in postmitotic cells and between 16 and 19 hr in serum-stimulated quiescent cells. A similar delay in the time of entry into S phase occurred after serum stimulation of resting cell populations. The results are compatible with the hypothesis that cells after mitosis may enter a state that is different from the state of cells made quiescent by serum restriction. |
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Authors:
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T Ashihara; F Traganos; R Baserga; Z Darzynkiewicz |
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Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Cancer research Volume: 38 ISSN: 0008-5472 ISO Abbreviation: Cancer Res. Publication Date: 1978 Aug |
Date Detail:
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Created Date: 1978-09-25 Completed Date: 1978-09-25 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 2984705R Medline TA: Cancer Res Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 2514-8 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Acridines Cell Cycle* Cells, Cultured Culture Media DNA / biosynthesis Fluorometry Interphase Mitosis* RNA / metabolism Time Factors |
| Chemical | |
Reg. No./Substance:
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0/Acridines; 0/Culture Media; 63231-63-0/RNA; 9007-49-2/DNA |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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