Document Detail


A comparison between oocyte growth in coculture with granulosa cells and oocytes with granulosa cell-oocyte junctional contact maintained in vitro.
MedLine Citation:
PMID:  512597     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Evidence is presented that strongly supports the hypothesis that the junctional association between oocytes and granulosa cells must be maintained to promote oocyte growth and development in vitro and that the coculture of oocytes with granulosa cells is not a sufficient condition for oocyte development. Furthermore, it is shown that incorporation of uridine and leucine by oocytes into TCA-insoluble material is significantly greater in granulosa cell-enclosed oocytes than in cocultured oocytes.
Authors:
J J Eppig
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Publication Detail:
Type:  Comparative Study; In Vitro; Journal Article; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  The Journal of experimental zoology     Volume:  209     ISSN:  0022-104X     ISO Abbreviation:  J. Exp. Zool.     Publication Date:  1979 Aug 
Date Detail:
Created Date:  1980-02-28     Completed Date:  1980-02-28     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0375365     Medline TA:  J Exp Zool     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  345-53     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Carbon Dioxide / metabolism
Cells, Cultured
Female
Granulosa Cells / physiology*
Intercellular Junctions / physiology*
Leucine / metabolism
Mice
Oocytes / growth & development*,  metabolism
Ovum / growth & development*
Pyruvates / metabolism
Uridine / metabolism
Chemical
Reg. No./Substance:
0/Pyruvates; 124-38-9/Carbon Dioxide; 58-96-8/Uridine; 61-90-5/Leucine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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