Document Detail


A comparison between the one-step and the two-step copper thiocholine procedure for the cytochemical localization of cholinesterases.
MedLine Citation:
PMID:  833016     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A comparison between the one-step and the two step copper thiocholine procedure for the subcellular localization of cholinesterases was undertaken. The results indicate that only under experimental conditions with short incubation times and precise control of the conversion into sulphide is the localization of the primary precipitate and that of the secondary precipitate identical. It was concluded that the conversion of the primary precipitate into Cu-sulphide is not necessary and can lead to artefacts.
Authors:
B Klinar; M Brzin
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Histochemistry     Volume:  50     ISSN:  0301-5564     ISO Abbreviation:  Histochemistry     Publication Date:  1977 Feb 
Date Detail:
Created Date:  1977-03-31     Completed Date:  1977-03-31     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0411300     Medline TA:  Histochemistry     Country:  GERMANY, WEST    
Other Details:
Languages:  eng     Pagination:  313-8     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Cholinesterases / analysis*
Copper
Histocytochemistry
Methods
Rats
Sulfides
Thiocholine
Chemical
Reg. No./Substance:
0/Sulfides; 625-00-3/Thiocholine; 7440-50-8/Copper; EC 3.1.1.8/Cholinesterases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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