| A comparison between the epidermal regenerative responses provoked by a skin irritant and a tumor promoter using anti-BrdUrd/DNA flow cytometry. | |
| | |
MedLine Citation:
|
PMID: 2029747 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
The proliferative responses induced in hairless mouse epidermis after application of the tumor promoter 12-O-tetradecanoylphorbol-13-acetate (TPA) and the skin irritant cantharidin were investigated. Doses known to give the same degree of hyperplasia were chosen. Mice were pulse-labeled with bromodeoxyuridine (BrdUrd) 30 min prior to or 24 h after a single application of either cantharidin or TPA, and thereafter killed at various time intervals. The basal cells were isolated from epidermis, fixed in 70% ethanol and prepared for bivariate BrdUrd/DNA flow cytometric analysis. Cells pulse-labeled in S phase 30 min prior to treatment with cantharidin or TPA were slightly delayed in their progression through S phase and temporarily blocked in G2 phase. However, they were still able to re-enter S phase 18 h later, indicating a shortening of the G1 phase. Cells pulse-labeled 24 h after treatment had a considerably reduced cell cycle time, i.e. reduced G1 transit time. Hence, the wave of cells entering S phase from 16 h after injury could be explained by an immediate reduction in G1 transit time, without assuming recruitment of temporarily resting G0/G1 cells. Although cantharidin caused the longest initial delay in cell cycle progression, the subsequent proliferative response was less pronounced than that provoked by TPA. Rapid proliferation may allow for clonal expansion of initiated cells. The higher ability of TPA to induce rapid proliferation, apparently without causing any severe initial cell damage, may thus be related to its higher tumor promoting ability. |
| | |
Authors:
|
B Kirkhus; W M Olsen; O P Clausen |
Related Documents
:
|
6992987 - Effects of 12-o-tetradecanoylphorbol-13-acetate on the differentiation of avian melanoc... 1303737 - A novel glycosignaling system: gq1b-dependent neuritogenesis of human neuroblastoma cel... 9745617 - Promotion-sensitive epidermal and mammary epithelial cells maintained in suspension ove... 10572947 - Activation of protein kinase c increases proteoglycan synthesis in immature rat sertoli... 15834587 - Heme oxygenase-1 accelerates protumoral effects of nitric oxide in cancer cells. 10460757 - Oxygen induces s-phase growth arrest and increases p53 and p21(waf1/cip1) expression in... |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: Carcinogenesis Volume: 12 ISSN: 0143-3334 ISO Abbreviation: Carcinogenesis Publication Date: 1991 May |
Date Detail:
|
Created Date: 1991-06-19 Completed Date: 1991-06-19 Revised Date: 2008-11-21 |
Medline Journal Info:
|
Nlm Unique ID: 8008055 Medline TA: Carcinogenesis Country: UNITED STATES |
Other Details:
|
Languages: eng Pagination: 833-7 Citation Subset: IM |
Affiliation:
|
Institute of Pathology, National Hospital, Oslo, Norway. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Bromodeoxyuridine* Carcinogens* Cell Cycle / drug effects DNA* Flow Cytometry Irritants* Mice Mice, Hairless Regeneration / drug effects* Skin / drug effects* Skin Physiological Phenomena Tetradecanoylphorbol Acetate / toxicity* |
| Chemical | |
Reg. No./Substance:
|
0/Carcinogens; 0/Irritants; 16561-29-8/Tetradecanoylphorbol Acetate; 59-14-3/Bromodeoxyuridine; 9007-49-2/DNA |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Induction of mutations by N-acetoxy-N-acetyl-2-aminofluorene modified M13 viral DNA.
Next Document: Thiol-enhanced decomposition of MNNG, ENNG, and nitrosocimetidine: relationship to mutagenicity in V...