| A comparison of aorta and vena cava medial message expression by cDNA array analysis identifies a set of 68 consistently differentially expressed genes, all in aortic media. | |
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MedLine Citation:
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PMID: 11009569 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We performed a systematic analysis of gene expression in arteries and veins by comparing message profiles of macaque aorta and vena cava media using a cDNA array containing 4048 known human genes, approximately 35% of currently named human genes (approximately 11,000). The data show extensive differences in RNA expression in artery versus vein media. Sixty-eight genes had consistent elevation in message expression by the aorta, but none were elevated in the vena cava. The most differentially expressed gene was regulator of G-protein signaling (RGS) 5, at an expression ratio of 46.5+/-12.6 (mean+/-SEM). The data set also contained 2 genes already known to be expressed in the aorta, elastin at 5.0+/-1.4, and the aortic preferentially expressed gene 1 (APEG-1) at 2.3+/-0.6. We chose to analyze RGS5 expression further because of its high level of differential expression in the aorta. Levels of RGS5 mRNA were confirmed by Northern analysis and in situ hybridization. A human tissue RNA dot blot showed that RGS5 message is highest in aorta, followed by small intestine, stomach, and then heart. Northern analysis confirmed that RGS5 expression in human aorta is higher than in any region of the heart. RGS5 is a G-protein signaling regulator of unknown specificity most homologous to RGS4, an inhibitory regulator of pressure-induced cardiac hypertrophy. The expression pattern of the 68 differential genes as a whole is a start toward identifying the molecular phenotypes of arteries and veins on a systematic basis. |
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Authors:
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L D Adams; R L Geary; B McManus; S M Schwartz |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Circulation research Volume: 87 ISSN: 1524-4571 ISO Abbreviation: Circ. Res. Publication Date: 2000 Sep |
Date Detail:
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Created Date: 2000-10-04 Completed Date: 2000-10-30 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 0047103 Medline TA: Circ Res Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 623-31 Citation Subset: IM |
Affiliation:
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Department of Pathology, University of Washington, Seattle, WA 98195-7335, USA. ladams@u.washington.edu |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adult Animals Aorta / physiology* Blotting, Northern Culture Media DNA Probes DNA, Complementary / analysis Female Gene Expression Regulation* Humans In Situ Hybridization Macaca Male Middle Aged Oligonucleotide Array Sequence Analysis RGS Proteins / genetics Rats Rats, Sprague-Dawley Venae Cavae / physiology* |
| Grant Support | |
ID/Acronym/Agency:
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P01HL03174/HL/NHLBI NIH HHS; R01HL 57557/HL/NHLBI NIH HHS; R01HL58083/HL/NHLBI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Culture Media; 0/DNA Probes; 0/DNA, Complementary; 0/RGS Proteins; 0/RGS5 protein, human; 0/Rgs5 protein, rat |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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