Document Detail


A comparison of the ability of beta-galactosidase and horseradish peroxidase enzyme-antibody conjugates to detect specific antibodies.
MedLine Citation:
PMID:  118216     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The ability of two different enzyme-antibody conjugates to detect specific antibodies has been compared. beta-Galactosidase was conjugated to antibodies raised against rabbit Fc fragments using m-maleimidobenzoyl-N-hydroxysuccinimide ester (MBS). Horseradish peroxidase (HRP) was conjugated to part of the same batch of antibodies using the periodate method. The beta-galactosidase and HRP labels enabled detection of approximately 8 fmoles and 4 fmoles respectively of human growth hormone (HGH) antibodies, when their enzyme activities were measured spectrophotometrically. The detection limit of the beta-galactosidase label was increased 4-fold when a fluorimetric detection system was employed.
Authors:
M J O'Sullivan; E Gnemmi; A D Simmonds; G Chieregatti; E Heyderman; J W Bridges; V Marks
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Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Journal of immunological methods     Volume:  31     ISSN:  0022-1759     ISO Abbreviation:  J. Immunol. Methods     Publication Date:  1979  
Date Detail:
Created Date:  1980-03-24     Completed Date:  1980-03-24     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  1305440     Medline TA:  J Immunol Methods     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  247-50     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Antibodies*
Antibody Specificity
Binding Sites, Antibody
Galactosidases / immunology*
Growth Hormone / immunology
Horseradish Peroxidase / immunology*
Humans
Immune Sera / pharmacology
Perissodactyla
Peroxidases / immunology*
Rabbits
beta-Galactosidase / immunology*
Chemical
Reg. No./Substance:
0/Antibodies; 0/Binding Sites, Antibody; 0/Immune Sera; 9002-72-6/Growth Hormone; EC 1.11.1.-/Horseradish Peroxidase; EC 1.11.1.-/Peroxidases; EC 3.2.1.-/Galactosidases; EC 3.2.1.23/beta-Galactosidase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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