Document Detail


A comparative study of three techniques for eluting red cell antibodies.
MedLine Citation:
PMID:  9347638     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Sixty-seven eluates obtained from the heat, ether and acid elution techniques were tested with the specific red blood cells (RBCs) and were compared according to their reactivities using the indirect antiglobulin test (IAT). It was found that the ether elution technique was superior in eluting Rh antibodies except for anti-e while the acid elution technique was superior in eluting Miltenberger (Mi(a)) antibodies (P < 0.05). The heat elution technique gave the lowest reactivity among the three techniques. In conclusion, the reactivities of the eluates obtained from the acid elution technique were overall comparable to those from the ether elution technique. The acid elution technique is practical for routine use in most blood banks because it is less time consuming and reduces the risk of exposing hazardous chemicals.
Authors:
O Nathalang; S Sthabunsawasdigarn; S Bejrachandra; J Saipin; T Sriphaisal
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Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Journal of the Medical Association of Thailand = Chotmaihet thangphaet     Volume:  80 Suppl 1     ISSN:  0125-2208     ISO Abbreviation:  J Med Assoc Thai     Publication Date:  1997 Sep 
Date Detail:
Created Date:  1997-12-23     Completed Date:  1997-12-23     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  7507216     Medline TA:  J Med Assoc Thai     Country:  THAILAND    
Other Details:
Languages:  eng     Pagination:  S5-8     Citation Subset:  IM    
Affiliation:
Department of Pathology, Pramongkutklao College of Medicine, Bangkok, Thailand.
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MeSH Terms
Descriptor/Qualifier:
Acids
Coombs' Test
Erythrocytes / immunology*
Ether, Ethyl
Hot Temperature
Humans
Isoantibodies / isolation & purification*
Methods
Chemical
Reg. No./Substance:
0/Acids; 0/Isoantibodies; 60-29-7/Ether, Ethyl

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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