| A comparative study of three techniques for eluting red cell antibodies. | |
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MedLine Citation:
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PMID: 9347638 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Sixty-seven eluates obtained from the heat, ether and acid elution techniques were tested with the specific red blood cells (RBCs) and were compared according to their reactivities using the indirect antiglobulin test (IAT). It was found that the ether elution technique was superior in eluting Rh antibodies except for anti-e while the acid elution technique was superior in eluting Miltenberger (Mi(a)) antibodies (P < 0.05). The heat elution technique gave the lowest reactivity among the three techniques. In conclusion, the reactivities of the eluates obtained from the acid elution technique were overall comparable to those from the ether elution technique. The acid elution technique is practical for routine use in most blood banks because it is less time consuming and reduces the risk of exposing hazardous chemicals. |
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Authors:
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O Nathalang; S Sthabunsawasdigarn; S Bejrachandra; J Saipin; T Sriphaisal |
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Publication Detail:
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Type: Comparative Study; Journal Article |
Journal Detail:
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Title: Journal of the Medical Association of Thailand = Chotmaihet thangphaet Volume: 80 Suppl 1 ISSN: 0125-2208 ISO Abbreviation: J Med Assoc Thai Publication Date: 1997 Sep |
Date Detail:
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Created Date: 1997-12-23 Completed Date: 1997-12-23 Revised Date: 2008-11-21 |
Medline Journal Info:
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Nlm Unique ID: 7507216 Medline TA: J Med Assoc Thai Country: THAILAND |
Other Details:
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Languages: eng Pagination: S5-8 Citation Subset: IM |
Affiliation:
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Department of Pathology, Pramongkutklao College of Medicine, Bangkok, Thailand. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Acids Coombs' Test Erythrocytes / immunology* Ether, Ethyl Hot Temperature Humans Isoantibodies / isolation & purification* Methods |
| Chemical | |
Reg. No./Substance:
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0/Acids; 0/Isoantibodies; 60-29-7/Ether, Ethyl |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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