Document Detail


A comparative study of the sulfation of bile acids and a bile alcohol by the Zebra danio (Danio rerio) and human cytosolic sulfotransferases (SULTs).
MedLine Citation:
PMID:  21839837     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The current study was designed to examine the sulfation of bile acids and bile alcohols by the Zebra danio (Danio rerio) SULTs in comparison with human SULTs. A systematic analysis using the fifteen Zebra danio SULTs revealed that SULT3 ST2 and SULT3 ST3 were the major bile acid/alcohol-sulfating SULTs. Among the eleven human SULTs, only SULT2A1 was found to be capable of sulfating bile acids and bile alcohols. To further investigate the sulfation of bile acids and bile alcohols by the two Zebra danio SULT3 STs and the human SULT2A1, pH-dependence and kinetics of the sulfation of bile acids/alcohols were analyzed. pH-dependence experiments showed that the mechanisms underlying substrate recognition for the sulfation of lithocholic acid (a bile acid) and 5α-petromyzonol (a bile alcohol) differed between the human SULT2A1 and the Zebra danio SULT3 ST2 and ST3. Kinetic analysis indicated that both the two Zebra danio SULT3 STs preferred petromyzonol as substrate compared to bile acids. In contrast, the human SULT2A1 was more catalytically efficient toward lithocholic acid than petromyzonol. Collectively, the results imply that the Zebra danio and human SULTs have evolved to serve for the sulfation of, respectively, bile alcohols and bile acids, matching the cholanoid profile in these two vertebrate species.
Authors:
Katsuhisa Kurogi; Matthew D Krasowski; Elisha Injeti; Ming-Yih Liu; Frederick E Williams; Yoichi Sakakibara; Masahito Suiko; Ming-Cheh Liu
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2011-08-04
Journal Detail:
Title:  The Journal of steroid biochemistry and molecular biology     Volume:  127     ISSN:  1879-1220     ISO Abbreviation:  J. Steroid Biochem. Mol. Biol.     Publication Date:  2011 Nov 
Date Detail:
Created Date:  2011-11-18     Completed Date:  2012-01-12     Revised Date:  2013-06-28    
Medline Journal Info:
Nlm Unique ID:  9015483     Medline TA:  J Steroid Biochem Mol Biol     Country:  England    
Other Details:
Languages:  eng     Pagination:  307-14     Citation Subset:  IM    
Copyright Information:
Copyright © 2011 Elsevier Ltd. All rights reserved.
Affiliation:
Department of Pharmacology, College of Pharmacy, The University of Toledo, Toledo, OH 43614, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Bile Acids and Salts / metabolism*
Cholestanols / metabolism*
Cytosol / enzymology*
Humans
Hydrogen-Ion Concentration
Kinetics
Substrate Specificity
Sulfates / metabolism*
Sulfotransferases / metabolism*
Zebrafish
Grant Support
ID/Acronym/Agency:
GM085756/GM/NIGMS NIH HHS; K08-GM074238/GM/NIGMS NIH HHS; R15 GM085756/GM/NIGMS NIH HHS
Chemical
Reg. No./Substance:
0/Bile Acids and Salts; 0/Cholestanols; 0/Sulfates; EC 2.8.2.-/Sulfotransferases
Comments/Corrections

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