| A comparative study of reducing the extracellular potassium concentration in red blood cells by washing and by reduction of additive solution. | |
| | |
MedLine Citation:
|
PMID: 17302770 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
BACKGROUND: Extracellular potassium concentration (K(+)) increases in the supernatant of whole and packed red blood cell units (pRBCs) with duration of refrigerated storage in citrate-phosphate-dextrose-adenine (CPDA-1) and additive solution (AS). Studies have shown that to avoid hyperkalemia, washed pRBCs are preferred if relatively fresh pRBCs are not available. To determine whether a simpler procedure, AS reduction, results in lowering of K(+) in pRBCs comparable to that achieved by washing, the K(+) levels by both methods were compared. STUDY DESIGN AND METHODS: Pre- and post-K(+) levels were measured in 6 washed and 11 AS-reduced pRBC units. Each unit was weighed, hematocrit was determined, K(+) was measured, and total K(+) was calculated. Washed units were 3 to 21 and AS-reduced units were 4 to 30 days old. Statistical analysis was performed with a t test. RESULTS: There was no significant difference (p > 0.35) in the initial K(+) between the two groups (mean +/- SD, 36.95 +/- 13.16 mEq/L before washing and 39.78 +/- 19.94 mEq/L before AS reduction). Washing and AS reduction both led to a significant decrease in K(+) levels (2.15 +/- 0.10 mEq/L after washing and 4.41 +/- 3.04 mEq/L after AS reduction, each p < 0.0005). Washing, however, was significantly better than AS reduction in reducing K(+) in stored pRBCs (p < 0.05). CONCLUSIONS: Washing pRBCs results in very low levels of K(+). AS reduction also significantly reduces K(+) levels. Selection of the method of K(+) reduction will depend on the stringency of K(+) reduction needed, the time constraints, and the availability of facilities and staff for washing. |
| | |
Authors:
|
Ila Bansal; Beverly W Calhoun; Cherilyn Joseph; Mohammad Pothiawala; Beverly W Baron |
Publication Detail:
|
Type: Comparative Study; Journal Article |
Journal Detail:
|
Title: Transfusion Volume: 47 ISSN: 0041-1132 ISO Abbreviation: Transfusion Publication Date: 2007 Feb |
Date Detail:
|
Created Date: 2007-02-16 Completed Date: 2007-03-27 Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 0417360 Medline TA: Transfusion Country: United States |
Other Details:
|
Languages: eng Pagination: 248-50 Citation Subset: IM |
Affiliation:
|
Department of Pathology (Blood Bank), The University of Chicago, Chicago, IL 60637, USA. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Blood Banks
/
methods Blood Preservation* Erythrocyte Transfusion / methods* Erythrocytes / metabolism* Humans Hyperkalemia / prevention & control* Potassium / blood* Solutions |
| Chemical | |
Reg. No./Substance:
|
0/Solutions; 7440-09-7/Potassium |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Inactivation of Orientia tsutsugamushi in red blood cells, plasma, and platelets with riboflavin and...
Next Document: Development of proficiency testing for detection of bacterial contamination of platelet products.