| A comparative study of the proteins of rat plasma, liver and hepatoma by agarose immunoelectrophoresis. | |
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MedLine Citation:
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PMID: 4340062 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A convenient and selective microtechnique, agarose immunoelectrophoresis, was applied to a comparison of the antigens in rat liver and plasma, and in primary hepatoma induced in male Fischer strain rats with N-2-fluorenylacetamide or N-hydroxy-N-2-fluorenylacetamide. Of the many soluble proteins antigenic in this system, 3 were singled out for detailed study. The albumin in liver and hepatoma had a higher mobility than that in plasma. Extraction of the soluble fraction of rat liver with ether, or treatment with absorbing charcoal, yielded an albumin band with a mobility identical to that in plasma, suggesting that liver albumin carries absorbed molecules with electronegative charges. The transferrin arc from liver, plasma and hepatoma had identical mobility. One protein with low mobility was present in higher concentration in the soluble liver fraction of male than of female rats, but it was reduced in hepatoma of male rats. The "h(2)" proteins of liver were found in the cathodic region as 5 arcs, some of which were reduced, while others were not detectable in hepatoma. |
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Authors:
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C Deckers; R M Glass; P H Grantham; R S Yamamoto; J H Weisburger |
Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: British journal of cancer Volume: 26 ISSN: 0007-0920 ISO Abbreviation: Br. J. Cancer Publication Date: 1972 Jun |
Date Detail:
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Created Date: 1972-09-21 Completed Date: 1972-09-21 Revised Date: 2011-07-20 |
Medline Journal Info:
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Nlm Unique ID: 0370635 Medline TA: Br J Cancer Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 190-200 Citation Subset: IM |
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| MeSH Terms | |
Descriptor/Qualifier:
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Absorption Acetamides Albumins / analysis Animals Antigens Antigens, Neoplasm Blood Protein Electrophoresis Blood Proteins / analysis* Carcinoma, Hepatocellular / chemically induced, immunology* Charcoal Ethyl Ethers Immunoelectrophoresis Liver / immunology* Liver Neoplasms Male Neoplasm Proteins / analysis* Polysaccharides Proteins / analysis* Rabbits / immunology Rats Serum Albumin / analysis Sex Factors Transferrin / analysis |
| Chemical | |
Reg. No./Substance:
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0/Acetamides; 0/Albumins; 0/Antigens; 0/Antigens, Neoplasm; 0/Blood Proteins; 0/Ethyl Ethers; 0/Neoplasm Proteins; 0/Polysaccharides; 0/Proteins; 0/Serum Albumin; 11096-37-0/Transferrin; 16291-96-6/Charcoal |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
| Full Text | |
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Journal Information Journal ID (nlm-ta): Br J Cancer ISSN: 0007-0920 ISSN: 1532-1827 |
Article Information Download PDF  Print publication date: Month: 06 Year: 1972 Volume: 26 Issue: 3 First Page: 190 Last Page: 200 ID: 2008471 PubMed Id: 4340062 PubMed Id: 4340062 |
| A Comparative Study of the Proteins of Rat Plasma, Liver and Hepatoma by Agarose Immunoelectrophoresis | |
| C. Deckers | |
| R. M. Glass | |
| P. H. Grantham | |
| R. S. Yamamoto | |
| J. H. Weisburger | |
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