Document Detail

A comparative study of extracellular matrix remodeling in two murine models of emphysema.
MedLine Citation:
PMID:  23275309     Owner:  NLM     Status:  In-Data-Review    
A single instillation of porcine pancreatic elastase (PPE) results in significant airspace enlargement on the 28th day after instillation, whereas cigarette smoke (CS) exposure requires 6 months to produce mild emphysema in rodents. Considering that there are differences in the pathogenesis of parenchymal destruction in these different experimental models, it is likely that there may be different patterns of extracellular matrix (ECM) remodeling. To evaluate ECM remodeling, C57BL/6 mice were submitted to either a nasal drop of PPE (PPE 28 Days) or exposed for 6 months to cigarette smoke (CS 6 months). Control groups received either an intranasal instillation of saline solution (Saline 28 Days) or remained without any smoke inhalation for six months (Control 6 months). We measured the mean linear intercept and the volume proportion of collagen type I, collagen type III, elastin and fibrillin. We used emission-scanning confocal microscopy to verify the fiber distribution. Both models induced increased mean linear intercept in relation to the respective controls, being larger in the elastase model in relation to the CS model. In the CS model, emphysema was associated with an increase in the volume proportion of fibrillin, whereas in the PPE model there was an increase in the parenchymal elastin content. In both models, there was an increase in collagen type III, which was higher in the CS-exposed mice. We concluded that ECM remodeling is different in the two most used experimental models of emphysema.
F D T Q S Lopes; A C Toledo; C R Olivo; C M Prado; E A Leick; M C Medeiros; A B G Santos; A Garippo; M A Martins; T Mauad
Related Documents :
22967129 - Modeling coding-sequence evolution within the context of residue solvent accessibility.
23857749 - Negative effects of climate warming on maize yield are reversed by the changing of sowi...
24752569 - Sample size considerations in prevention research applications of multilevel modeling a...
23004929 - Preclinical antitumor activity of thiarabine in human leukemia and lymphoma xenograft m...
11162299 - Features of the value function for voice and their consistency across participants from...
23850209 - Effects of nom properties on copper release from model solid phases.
25403719 - The geko™ electro-stimulation device for venous thromboembolism prophylaxis: a nice m...
23785219 - Fractals with point impact in functional linear regression.
9194269 - Testing for treatment differences with dropouts present in clinical trials--a composite...
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Histology and histopathology     Volume:  28     ISSN:  1699-5848     ISO Abbreviation:  Histol. Histopathol.     Publication Date:  2013 Feb 
Date Detail:
Created Date:  2012-12-31     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8609357     Medline TA:  Histol Histopathol     Country:  Spain    
Other Details:
Languages:  eng     Pagination:  269-76     Citation Subset:  IM    
Department of Medicine, School of Medicine, University of Sao Paulo, Brazil.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Rat hair follicle-constituting cells labeled by a newly-developed somatic stem cell-recognizing anti...
Next Document:  Aberrant expression and association of VEGF and Dll4/Notch pathway molecules under hypoxia in patien...