Document Detail


A comparative study of the effect of cholesterol on bicelle model membranes using X-band and Q-band EPR spectroscopy.
MedLine Citation:
PMID:  19501076     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
X-band and Q-band electron paramagnetic resonance (EPR) spectroscopic techniques were used to investigate the structure and dynamics of cholesterol containing phospholipid bicelles based upon molecular order parameters (S(mol)), orientational dependent hyperfine splittings and line shape analysis of the corresponding EPR spectra. The nitroxide spin-label 3-beta-doxyl-5-alpha-cholestane (cholestane) was incorporated into DMPC/DHPC bicelles to report the alignment of bicelles in the static magnetic field. The influence of cholesterol on aligned phospholipid bicelles in terms of ordering, the ease of alignment, phase transition temperature have been studied comparatively at X-band and Q-band. At a magnetic field of 1.25 T (Q-band), bicelles with 20 mol% cholesterol aligned at a much lower temperature (313 K), when compared to 318 K at a 0.35 T field strength for X-band, showed better hyperfine splitting values (18.29 G at X-band vs. 18.55 G at Q-band for perpendicular alignment and 8.25 G at X-band vs. 7.83 G at Q-band for the parallel alignment at 318 K) and have greater molecular order parameters (0.76 at X-band vs. 0.86 at Q-band at 318 K). Increasing cholesterol content increased the bicelle ordering, the bicelle-alignment temperature and the gel to liquid crystalline phase transition temperature. We observed that Q-band is more effective than X-band for studying aligned bicelles, because it yielded a higher ordered bicelle system for EPR spectroscopic studies.
Authors:
Harishchandra Ghimire; Johnson J Inbaraj; Gary A Lorigan
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, Non-P.H.S.     Date:  2009-06-06
Journal Detail:
Title:  Chemistry and physics of lipids     Volume:  160     ISSN:  1873-2941     ISO Abbreviation:  Chem. Phys. Lipids     Publication Date:  2009 Aug 
Date Detail:
Created Date:  2009-07-07     Completed Date:  2009-10-22     Revised Date:  2013-06-02    
Medline Journal Info:
Nlm Unique ID:  0067206     Medline TA:  Chem Phys Lipids     Country:  Ireland    
Other Details:
Languages:  eng     Pagination:  98-104     Citation Subset:  IM    
Affiliation:
Department of Chemistry and Biochemistry, Miami University, 701 E. High Street, Oxford, OH 45056, USA.
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MeSH Terms
Descriptor/Qualifier:
Cholesterol / chemistry*
Dimyristoylphosphatidylcholine / chemistry
Electron Spin Resonance Spectroscopy / methods*
Lipid Bilayers / chemistry*
Liquid Crystals / chemistry
Magnetics
Membranes, Artificial
Phase Transition
Phospholipid Ethers / chemistry
Transition Temperature
Grant Support
ID/Acronym/Agency:
GM60259-01/GM/NIGMS NIH HHS; R01 GM080542-01A2/GM/NIGMS NIH HHS; R01 GM080542-05/GM/NIGMS NIH HHS
Chemical
Reg. No./Substance:
0/1,2-dihexadecyl-sn-glycero-3-phosphocholine; 0/Lipid Bilayers; 0/Membranes, Artificial; 0/Phospholipid Ethers; 13699-48-4/Dimyristoylphosphatidylcholine; 57-88-5/Cholesterol
Comments/Corrections

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