| A comparative study of the effect of cholesterol on bicelle model membranes using X-band and Q-band EPR spectroscopy. | |
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MedLine Citation:
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PMID: 19501076 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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X-band and Q-band electron paramagnetic resonance (EPR) spectroscopic techniques were used to investigate the structure and dynamics of cholesterol containing phospholipid bicelles based upon molecular order parameters (S(mol)), orientational dependent hyperfine splittings and line shape analysis of the corresponding EPR spectra. The nitroxide spin-label 3-beta-doxyl-5-alpha-cholestane (cholestane) was incorporated into DMPC/DHPC bicelles to report the alignment of bicelles in the static magnetic field. The influence of cholesterol on aligned phospholipid bicelles in terms of ordering, the ease of alignment, phase transition temperature have been studied comparatively at X-band and Q-band. At a magnetic field of 1.25 T (Q-band), bicelles with 20 mol% cholesterol aligned at a much lower temperature (313 K), when compared to 318 K at a 0.35 T field strength for X-band, showed better hyperfine splitting values (18.29 G at X-band vs. 18.55 G at Q-band for perpendicular alignment and 8.25 G at X-band vs. 7.83 G at Q-band for the parallel alignment at 318 K) and have greater molecular order parameters (0.76 at X-band vs. 0.86 at Q-band at 318 K). Increasing cholesterol content increased the bicelle ordering, the bicelle-alignment temperature and the gel to liquid crystalline phase transition temperature. We observed that Q-band is more effective than X-band for studying aligned bicelles, because it yielded a higher ordered bicelle system for EPR spectroscopic studies. |
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Authors:
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Harishchandra Ghimire; Johnson J Inbaraj; Gary A Lorigan |
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Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, Non-P.H.S. Date: 2009-06-06 |
Journal Detail:
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Title: Chemistry and physics of lipids Volume: 160 ISSN: 1873-2941 ISO Abbreviation: Chem. Phys. Lipids Publication Date: 2009 Aug |
Date Detail:
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Created Date: 2009-07-07 Completed Date: 2009-10-22 Revised Date: 2011-08-01 |
Medline Journal Info:
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Nlm Unique ID: 0067206 Medline TA: Chem Phys Lipids Country: Ireland |
Other Details:
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Languages: eng Pagination: 98-104 Citation Subset: IM |
Affiliation:
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Department of Chemistry and Biochemistry, Miami University, 701 E. High Street, Oxford, OH 45056, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Cholesterol
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chemistry* Dimyristoylphosphatidylcholine / chemistry Electron Spin Resonance Spectroscopy / methods* Lipid Bilayers / chemistry* Liquid Crystals / chemistry Magnetics Membranes, Artificial Phase Transition Phospholipid Ethers / chemistry Transition Temperature |
| Grant Support | |
ID/Acronym/Agency:
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GM60259-01/GM/NIGMS NIH HHS; R01 GM080542-01A2/GM/NIGMS NIH HHS; R01 GM080542-05/GM/NIGMS NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/1,2-dihexadecyl-sn-glycero-3-phosphocholine; 0/Lipid Bilayers; 0/Membranes, Artificial; 0/Phospholipid Ethers; 13699-48-4/Dimyristoylphosphatidylcholine; 57-88-5/Cholesterol |
| Comments/Corrections | |
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